1.True or false: In a bacterial species with a "lac operon", the repressor protein will be bound to DNA when lactose is available to the bacterium 2. True or false: DNA replication follows a dispersive model 3.True or false: Morgan's fruit fly work showed that genes are made of DNA 4. True or false: If there is no lactose present in the cell of a bacterium with a "lac operon" the repressor protein will be bound to DNA

1.True or false: In a bacterial species with a "lac operon", the repressor protein will be bound to DNA when lactose is available to the bacterium 2. True or false: DNA replication follows a dispersive model 3.True or false: Morgan's fruit fly work showed that genes are made of DNA 4. True or false: If there is no lactose present in the cell of a bacterium with a "lac operon" the repressor protein will be bound to DNA

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 2TYU

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1. A strain of E. coli is genetically engineered in which the lacZ and lacY removed and replaced with a gene encoding a fluorescent protein. In addition, scientist produced a partial diploid E. coli strain, i.e. bacteria that have one full copy of the lac operon in their chromosome plus another copy of the lac operon in a plasmid. The modified bacteria have the follow genetic components: genes are One copy of the operon in which the protein product fluoresces green is inserted into the bacteria chromosome One copy of the operon in which the protein product fluoresces red is inserted into a plasmid. Both copies of the lac operon carry one copy of wild-type Promoter (lacP) and Operator (lacO) cis-acting DNA sequences. The modified bacteria have only one copy of the lacI repressor gene in their chromosomes. The lac operon in the plasmid doesn't have the lacI gene. If both fluorescent proteins are expressed, the cells fluoresce yellow and if neither of the fluorescent proteins is…
1. The lactose operon is controlled by both lactose and glucose. Fill in the following table to indicate what would be occurring under different cellular conditions Lactose absent present absent present Glucose Operon Activity (high/low) absent absent present present low high 10W high CAMP Repressor (bound/free) (high/low) bound free buind will high high low CAP (bound/free) bound free full
Which of the following is least related to the other items? Group of answer choices inducer repressor operator enhancers 2. A scientist observing a cell during gene expression would be able to easily distinguish it as a prokaryotic cell by which of the following observations? Group of answer choices as soon as the DNA introns are removed from the template after the 5' caps are converted to mRNA after a transcription initiation complex has been formed during transcription once the pre-mRNA has been converted to mRNA
In bacteria sigma factors: 1. Transcript factors2. Specialized subunit of the core enzyme 3. Signal termination 4. Guide RNA polymerase to different kinds of promoters
7) In a bacterial species with a "lac operon", the repressor protein will be bound to DNA when lactose is available to the bacterium True False
Escherichia coli is grown in the presence of 20 mg of glucose and 20mg of lactose as carbon sources. The major carbon source is consumed by Escherichia at a rate of 10 mg/hour. Which of the following best describes the actions of RNA polymerase, the promoter and the sugar being used after 3 hours? O The sugar binds to the repressor, inactivating it, allowing RNA polymerase to bind to the operator. O RNA polymerase binds to the promoter and the sugar binds to the operator. O The sugar binds to the repressor, inactivating it, and allows RNA polymerase to bind to the promoter. O The sugar binds to RNA polymerase and the repressor binds to a structural gene. Question 41 A Moving to the next question prevents changes to this answer. 61
Which of the following is characteristic of genes and gene regulation in both bacteria and eukaryotes? (a) promoters (b) non-coding DNA within coding sequences (c) enhancers (d) operons (e) DNA located in a nucleus
All of the following statements about the repressor of the lac operon of E. coli are true EXCEPT which one? O A. The repressor is the product of a regulatory gene i The repressor binds to the operator region of the DNA of the lac OB. operon. The repressor is a protein. OC. The repressor interacts with the operator in such a way as to OD. prevent translation of beta-galactosidase. The repressor can combine with lactose to form a OE. complex that will no longer bind to the operator region. QUESTION 32 The amino acid which is a precursor of serotonin is Histidine OA. O B. Tyrosine O C. Glutamate D. Tryptophan O E. none of the above
3. The activation of a gene is often regulated via a negative feedback loop using a repressor. Gene A synthesizes a small molecule called "Tonellase" that breaks down “Tonellin,” a rare nutrient. Promoter Operator Gene A RNA Polymerase Explain how the following operon would function if it was: a. Inducible (like the Lac Operon) b. Repressible (like the Trp Operon)
Operons are a common feature of bacterial genomes. Which of the following statements about the lac operon is correct? 1. The lac operon contains 3 stop codon sequences. 2. The lacz gene codes for lactose which is used as a source of energy. 3. A lacls mutant that can't bind to allolactose will inhibit the transcription of lacz, lacY and lacA. 4. A lacOC mutant that keeps the operator constitutively ON (regardless of repressor binding), will inhibit the transcription of lacZ, lacY and lacA. O A. 1, 2 and 3 O B. 1 and 3 O C. 2 and 4 O D. 4 only O E. All of 1, 2, 3 and 4 are correct
1. Assuming that the level of glucose is low, a mutation in the repressor associated with the lac operon of E. coli which prevents binding of the repressor to lactose should result in: a. constitutive expression of the lac operon genes b. lack of expression or refuced expression of the lac operon genes under all circumstances c. expression of the genes only when lactose is present d. expression of the genes only when lactose is absent e. lack of expression of the lac operon genes, resulting in an increase in lactose production
5. Regulation of bacterial operons by inducers, e.g. lactose, exhibits which of the following properties? Inducer effect on RNA polymerase binding to the promoter Inducer binds to the Repressor produced by: repressor and: Activates the repressor A. Activates the repressor A separate gene Genes of the operon A separate gene Genes of the operon A separate gene Enhances В. Enhances Activates the repressor D. Inhibits the repressor Inhibits the repressor С. Inhibits inhibits Е. No effect 6. Gene transcription rates and mRNA levels were determined for an enzyme that is induced by a newly developed drug. Compared with untreated levels, drug treatment caused a 10x increase in the gene transcription rate and a 20× increase in both mRNA levels and enzyme activity. These data indicate that a primary effect of the drug treatment is to decrease which one of the following? A. The activity of RNA polymerase B. The ability of miRNA to act on mRNA C. The rate of translation D. The rate of binding of…