1. The peptide below was isolated from fermented milk and shown to have antioxidant properties Lactobacillus delbrueckii subsp., bulgaricus IFO13953 N-Ala-Arg-His-Pro-His-Pro-His-Leu-Ser-Phe-Met -C a) Draw the primary structure of the from the N-terminal to the C-terminal G. Make sure that the ends and the R groups are in the ionization state that would be expected at biological pH=7. b) Place a box around each of the peptide bonds. c) Write the sequence using 1 letter abbreviations. d) What is the net charge of the peptide at pH=7? Calculate the pl. e) Draw the titration curve for the polypeptide and show where the pl and buffering regions are.. f) Identify -R groups that: Are hydrophobic, hydrophilic and aromatic • Are the H-bond donors and acceptors by placing a d or an a by the appropriate atom • can take part in electrostatic interactions. For the R groups that are charged at pH=7, list the other possible charged R groups that would interact favorably with the charged group in the peptide. (these should be chosen from the list of all common amino acids) 9) Explain how the pKa of an amino acid can differ depending on its chemical environment, either aqueous or hydrophobic. Consider both and acidic and basic amino acid in your explanation. (one paragraph).
1. The peptide below was isolated from fermented milk and shown to have antioxidant properties Lactobacillus delbrueckii subsp., bulgaricus IFO13953 N-Ala-Arg-His-Pro-His-Pro-His-Leu-Ser-Phe-Met -C a) Draw the primary structure of the from the N-terminal to the C-terminal G. Make sure that the ends and the R groups are in the ionization state that would be expected at biological pH=7. b) Place a box around each of the peptide bonds. c) Write the sequence using 1 letter abbreviations. d) What is the net charge of the peptide at pH=7? Calculate the pl. e) Draw the titration curve for the polypeptide and show where the pl and buffering regions are.. f) Identify -R groups that: Are hydrophobic, hydrophilic and aromatic • Are the H-bond donors and acceptors by placing a d or an a by the appropriate atom • can take part in electrostatic interactions. For the R groups that are charged at pH=7, list the other possible charged R groups that would interact favorably with the charged group in the peptide. (these should be chosen from the list of all common amino acids) 9) Explain how the pKa of an amino acid can differ depending on its chemical environment, either aqueous or hydrophobic. Consider both and acidic and basic amino acid in your explanation. (one paragraph).
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter9: Gene Expression And Gene Regulation
Section: Chapter Questions
Problem 15QP
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