1. How many NADH _____& ATP _____are produced from the beta oxidation of Lauric Acid? 2. How many FADH2 _____& ATP _____are produced from the beta oxidation of Lauric Acid?
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1. How many NADH _____& ATP _____are produced from the beta oxidation of Lauric Acid?
2. How many FADH2 _____& ATP _____are produced from the beta oxidation of Lauric Acid?
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- How many NADH _____& ATP _____are produced from the beta oxidation of Lauric Acid?2. During glycolysis, glucose is converted to pyruvate. Pyruvate then is converted to Acetyl coA and enters the citric acid cycle. Per one (1) glucose molecule that undergoes glycolysis and TCA cycle, complete the table below Equivalence in ATP Products from glycolysis 1. ATP 2. NADH 3. Pyruvate Products from TCA 4. NADH 5. FADH2 6. GTP TOTAL ATP Produced ATPS Equivalence in ATP ATPS ATPS ATPS ATPs produced ATP ATP = ATPs produced ATP ATP ATP 7._ATP1. True / False: NADH dehydrogenase is the least electronegative complex of the ETC. 2. True / False: Most of the energy lost during the conversion of one form to another is as light. 3. True / False: The addition of a phosphate group is called phosphorylation. 4. True / False: The ETC pumps protons into the mitochondrial matrix. 5. True / False: FADH₂ transfers its electrons directly to cytochrome c. 6. True / False: FADH₂ is created exclusively during the citric acid cycle. 7. True/False: Alcohol fermentation occurs most commonly in animals and plants. 8. True / False: During fermentation, NAD* is regenerated when NADH is reduced. 9. True / False: The hydrolysis of ATP releases free energy. 10. True / False: Most of the CO₂ produced by cellular respiration is formed during ETC/chemiosmosis.
- 3. The overall result of CH OH + 2 NAD+ O + 2 NADH glycolysis can be summarized by the equation on the right in HO- which the glucose origins of the carbon atoms in pyruvate are color coded. Decide which gly- colytic reactions are responsible for the indicated distribution and HO- + 2 Pi 2 + 2 ATP Но OH + 2 ADP CH3 + 2 H2O Glucose Pyruvate conversion of glucose atoms into pyruvate atoms Start by numbering of carbons in Glucose-6-Pi and Fructose-1,6-BisPi.6. Fill in the blanks and select the correct option [A/B/C] in the paragraph below. Although both NADH and FADH₂ bring high-energy electrons to the ETC, ultimately they produce different amounts of [ H₂O / ATP /0₂]. NADH is [oxidized / reduced] by NADH reductase resulting in 2 H*/protons being pumped from the to the [2/4/6] FADH₂ gives its electrons to [ ubiquinone / cytochrome c] which means that only [2/4/6] H*/protons are pumped. All electrons are eventually accepted by which produces water/H₂O. Ultimately, each NADH is responsible for the production of about [2/3 /4] ATP while FADH₂ makes [2/3/4] when the potential energy of the electrochemical gradient moves [ protons/electrons ] back into the matrix using the enzyme2. During glycolysis, glucose is converted to pyruvate. Pyruvate then is converted to Acetyl coA and enters the citric acid cycle. Per one (1) glucose molecule that undergoes glycolysis and TCA cycle, complete the table below Equivalence in ATP Products from glycolysis 1. ATP 2. _NADH Pyruvate ATP: produced ATP АТР ATPS 3. ATPS produced АТР АТР Products from TCA Equivalence in ATP NADH 5. _FADH2 6. GTP 4. ATPS ATPS ATPS АТР TOTAL ATP Produced 7. ATP
- Lactate dehydrogenase (LDH) plays an essential role in an exercising muscle, especially when the level of excursion surpasses the ability of circulatory systems ability to supply adequate oxygen. What is the main reason LDH is so important to the muscle under these conditions? It produces one mole of ATP via substrate level phosphorylation. It resupplies NAD+ to glyceraldehyde 3-phosphate dehydrogenase. It decreases pH thus decreasing the dissociation rate of oxygen from hemoglobin It produces NADH for energy while converting pyruvate into lactate. It removes excess lactic acid from the muscle to create pyruvate. Next1. Identify the oxidized coenzyme (letter abbreviation only) that participates in this reaction of the Kreb’s cycle. Succinate --> Fumarate 2. How many mol of NADH can be obtained upon the beta oxidation of stearic acid? 3. How many mol of ATP can be obtained upon the complete oxidation of 1 mol stearic acid? 4. How many steps in glycolysis in which ATO is converted to ADP?2a. How many NADH are made during glycolysis from one glucose? 2b. How many NADH are made during the citric acid cycle from one glucose? 2c. How many FADH2 are made during the citric acid cycle from one glucose?
- How many NADH _____& ATP _____are produced from the beta oxidation of Lauric Acid?o 4 NADH, 10 ATPo 5 NADH, 12.5 ATPo 6 NADH, 15 ATPo 6 NADH, 18 ATP2. Lactate dehydrogenase (LDH) catalyzes the reaction Ο ()) 0 NADH + H* NAD+ C=0 HO-C-H CH₁t Pyruvate lactate dehydrogenase CHa L-Lactate AG 25.1 kJ/mol which represents one of the metabolic fates of pyruvate, the end product of glycolysis. The positioning of the sub-strate pyruvate in the active site of lactate dehydrogenase is shown on the right. NADH (nicotinamide adenine dinu- cleotide) is a cofactor in the reaction and provides a hydride anion H (highlighted with light blue) through direct transfer to reduce the carbonyl group of pyruvate. (a) ( Gln 102 Arg109 NH2 NH Thr246 H₂C-C-OH HN NH H CHS H H H. His 195 NH Pyruvate N-(NADH) H H CH3 H HN H NH H₂C-C-CH₂ Пle250 Asp168 NH Arg171 ) Compare the mechanism of the LDH reaction, as implied by the diagram above, to that of a-chymotrypsin with respect to the oxyanion hole, conversion of the substrate carbonyl group having sp² hybridization to sp³ hybridization, the attacking nucleophile, and residues achoring the substrate in the active…3. The "actual" free-energy change, AG, for the oxidation of NADH under aerobic conditions is -50.9 kcal mol- when oxygen pressure is 10-2 atm. What is the ratio of NAD*/NADH under these conditions?