1. Determining Km and Vmax- You have recently discovered a new enzyme homeworkase which destroysyour homework assignments. In order to characterize this enzyme, you wish to determine the Km and Vmax.To do so, you make up several different concentrations of substrate (keeping the enzyme concentrationconstant) and run the reaction. You obtain the following results.[Substrate] (pages/liter)reaction rate (pages/liter/min)651.0 x 10-³5.0 x 10-41.0 x 1045.0 x 10.53.0 x 10-52.0 x 10-51.0 x 10-55.0 x 10€1.0 x 10€5.0 x 10-76351423327179.52.21.1(a) Is this data consistent with Michaelis Menten kinetics? (HINT: plot the data). Do not forget to includeyour well-labeled graph/plot!(b) Determine the Vmax and Km for this enzyme using (i) Lineweaver-Burk and an (ii) Eadie-Hofstee plot(you will have to look up the latter). How do the results compare with each other? Do not forget toinclude your well-labeled plots!

The rate equation for Michaelis Menten equation is given as, R= K2SES +KM where,S = substrate…

1. Determining Km and Vmax. You have recently discovered a new enzyme homeworkase which destroys your homework assignments. In order to characterize this enzyme, you wish to determine the Km and Vmax To do so, you make up several different concentrations of substrate (keeping the enzyme concentration constant) and run the reaction. You obtain the following results. [Substrate] (pages/liter) reaction rate (pages/liter/min) 65 63 51 1.0 x 10³ 5.0 x 104 1.0 x 10-4 5.0 x 105 3.0 x 10-6 2.0 x 105 1.0 x 105 5.0 x 10€ 1.0 x 10€ 5.0 x 10-7 42 33 27 17 9.5 2.2 1.1 (a) Is this data consistent with Michaelis Menten kinetics? (HINT: plot the data). Do not forget to include your well-labeled graph/plot! (b) Determine the Vmax and Km for this enzyme using (i) Lineweaver-Burk and an (ii) Eadie-Hofstee plot (you will have to look up the latter). How do the results compare with each other? Do not forget to include your well-labeled plots!

1. Determining Km and Vmax. You have recently discovered a new enzyme homeworkase which destroys your homework assignments. In order to characterize this enzyme, you wish to determine the Km and Vmax To do so, you make up several different concentrations of substrate (keeping the enzyme concentration constant) and run the reaction. You obtain the following results. [Substrate] (pages/liter) reaction rate (pages/liter/min) 65 63 51 1.0 x 10³ 5.0 x 104 1.0 x 10-4 5.0 x 105 3.0 x 10-6 2.0 x 105 1.0 x 105 5.0 x 10€ 1.0 x 10€ 5.0 x 10-7 42 33 27 17 9.5 2.2 1.1 (a) Is this data consistent with Michaelis Menten kinetics? (HINT: plot the data). Do not forget to include your well-labeled graph/plot! (b) Determine the Vmax and Km for this enzyme using (i) Lineweaver-Burk and an (ii) Eadie-Hofstee plot (you will have to look up the latter). How do the results compare with each other? Do not forget to include your well-labeled plots!

Chemistry

9th Edition

ISBN:9781133611097

Author:Steven S. Zumdahl

Publisher:Steven S. Zumdahl

Chapter12: Chemical Kinetics

Section: Chapter Questions

Problem 5RQ: Consider the zero-, first-, and second-order integrated rate laws. If you have concentration versus...

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Similar questions

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