1) both streak plating and pour plating produce isoluated colonies. What is the underlying explanation for why both methods work; that is, what are both methods doing with repect to the bacterial cells? 2) If streak plates failed to produce isolated colonies, describe two things that you could do to improve your chance of generating isolated colonies. 3) Why do we care so much about producing isolated colonies? what is an isolated colony composed of? What can you do with an isolated colony?

Q: Does each colony represent a pure colony? How will you prove your answer? Explain why an isolated…

A: A "colony" is a collection of bacteria, fungus, and other microbes cultivated on a solid agar media…

Q: in loop streak method ,student inoculate unknown bacteria      how many loop this method need ?…

A: The loop streak method is the rapid method of qualitative isolation of discrete colonies. In this…

Q: hat is the advantage of culture-independent analysis over culture-dependent analysis of…

A: An unknown microorganism can be identified and classified in a variety of ways. They are divided…

Q: Why is the level of contamination measured as number of colonies rather than size of colonies?

A: Microbiological contamination refers to the presence of unwanted or accidental introduction of…

Q: Why does a streaking method used to inoculate plates result in isolated colonies?

A: Streaking method is used to obtain pure colonies of microbes(bacteria) from a proper single species…

Q: What is the correct shape of the unknown bacterium growing on your MacConkey agar?

A: Macconkey Agar This agar includes bile salts, which are found in the gut. Bile salts help in…

Q: , the most suitable description for the origin of each colony formed on the surface of a solid…

A: Origin of colony in solid medium.

Q: student is directed to make a simple stain of coli with crystal violet, but got mixed up and used…

A: Bacteria cell walls are stained by the crystal violet. Iodine is subsequently added as a mordant to…

Q: Do we have to do an antibiotic sensitivity test to ensure the result? and what is the type and name…

A: Antibiotic Sensitivity Test:  Antibiotic sensitivity testing, often known as antibiotic…

Q: What are Koch's postulates? Discuss the importance of these postulates in the identification of…

A: As per Germ theory of Disease , pathogen ( disease causing agents) are the chief factors behind a…

Q: Why is it necessary to perform an enrichment procedure in isolating Staphylococcus aureus instead of…

A: Staphylococcus aureus is a Gram-positive round-shaped bacterium that is normally found in the upper…

Q: Is it acceptable to give a formal name to a microbe that hasn't been isolated and cultivated? What…

A: There are two main microbiology methods used for isolating bacteria from a clinical sample in order…

Q: Based on the same two strains of E. coli from the previous question, why do you see growth in the…

A: Ampicillin is a penicillin type of antibiotic substances containing medium So the  strain II can…

Q: You measure your E. coli and find them to have an OD600 of 1.20. You have 50 ml of culture, and you…

A: The growth curve of a bacterial culture can be assessed by measuring its optical density at 600 nm…

Q: Based on the results of the Kirby-Bauer test shown below, what can we say about this strain of…

A: In Kirby-Bauer testing, bacteria are put on a plate of solid growth medium and wafers of antibiotics…

Q: You prepared another slide to visualize the bacteria under the oil immersion objective lens, but you…

A: In microscopy, oil immersion is a technique used to increase the resolving power of a microscope.…

Q: 1 mL of a juice sample is taken and diluted in 9 mL culture medium, subsequently, this suspension is…

A: Petroff- Hausser chamber is a chamber used usually for sperm and bacterial count. Number of bacteria…

Q: If you plated 0.1mL of a 1:100 dilution of phage stock onto a plate with an E.coli lawn, what is…

A: In microbiology lab dilution factor represent the solute vs solvent ratio for making the the culture…

Q: Plaque assay (Bacteriophage Titer) Answer questions 2 & 3 2. Why don't bacteriophages continue…

A: Plaque assay is one of the widely used approaches for determining the quantity of infectious virus…

Q: Which bacterium produced a yellow halo, and how do you interpret this result? •  If you grew…

A: Microbes require micronutrients and macronutrients for growth and multiplication. Required nutrients…

Q: Why do serial dilution? Does serial dilution make it easier to count bacteria on a spread/streak…

A: Dilution is the process of making a solution weaker or less concentrated.

Q: Why the synergy test results demonstrated that Penicillin G and Gentamicin did not act…

A: In the development of antibacterial drugs, when two or more drugs are tested together…

Q: You have two strains of E. coli, Strain I and Strain II. These strains were also co- cultured on a…

A: Antibiotics are those substances that kill or prevent the growth of bacteria. Antibiotics can be of…

Q: If SIM medium was used for motility determination for Proteus vulgaris, what noticeable change to…

A: What is motility? Motility is the ability of an organism to move by itself. Eucaryotic cells use…

Q: Explain in your own words how bacteria are grown to form the sample for the antibiotic resistance…

A: Antibiotic resistance The term antibiotic resistance is made up of two words i.e. resistance(ability…

Q: Which of the following agents if introduced into a growing culture of bacteria would halt growth but…

A: INTRODUCTION Bacteria, also called germs, are microscopic organisms not visible with the naked eye.…

Q: Describe ,A Hfr bacterium mates with an F- plasmid containing bacterium. What

A: PLASMIDS- It was discovered that maleness in bacteria is determined by a transmissible genetic…

Q: how does the use of the streak plate technique enables us to isolate and purify bacteria

A: The streak plate technique is the popular method or technique to isolate and purify the bacteria.

Q: Is it likely that a pure culture could be isolated from the human body? Why or why not?

A: In microbiology the pure culture is a laboratory culture that contains a single species of organism.…

Q: You use a spectrophometer and estimate the concentration of bacteria in a urine sample to be 4.6 x…

A: dilution factor  =10^-6

Q: You are attempting to propagate bacteriophage of Bacillus cereus using a liquid batch culture. A…

A: Bacteriophage : these are the viruses that attack on barterial cells and use them to produce more…

Q: When using the blue/ white colony screening method, what do blue colonies represent ?

A: Blue- white screening is used for the identification of recombinant bacteria.

Q: Why is there an inverse relationship between the dilution factor and the amount of virus in…

A: The dilution factor is the calculation of how much a new solution is less concentrated than the…

Q: If Microbe A and Microbe B have whole genome similarity of 68%, as determined by DNA-DNA…

A: DNA full form is deoxyribonucleic acid. DNA is the main constituent of the chromosome. It contains…

Q: What colony characteristics can be used for differentiation of bacterial species? As an example,…

A: Colony morphology is the term used to describe the characteristics of an individual colony of…

Q: Assume that you counted 67 plaques on a bacterial plate where 0.1ml of a 10-5 dilution of phage was…

A: A dilution: It can be defined as a method of addition of more solvent to a solution of a definite…

Q: Electro blotting techniques are routinely used in diagnostic microbiology for detection of a…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: erial slide agglutination technique important in diagnostic procedure?

A: Immunoassays are bioanalytical ways that use the specificity of an antigen-antibody reaction to…

Q: Bacteria in the phylum Firmicutes are distinguished from the phylum Actinobacteria on the basis of…

A: A microbe is a living entity that is so tiny that it cannot be seen with the naked eye. Microbiology…

Q: What distinguishes Acid-Fast bacteria in terms of cell wall / coating and other characteristics?…

A: Acid-fast bacteria It is the acid-fast bacilli. These are the bacterial group that has the…

Q: When developing the zymogram using a destaining solution, a researcher observed wedge shaped bands…

A: Zymography is a technique to assess the enzymatic activity of proteins either in situ or by…

Q: Where/ How did the researchers find the bacterium, which they named Ideonella sakaiensis 201-F6?…

A: Ideonella sakaiensis is a unique bacterial species that is able to break down plastic and it is…

Q: E.coli was incubated with aeration in a nutrient medium containing two carbon sources provided one…

A: First of all, E. coli can utilize glucose and lactose both as a carbon source. Lactose is a…

Q: How do we isolate culture media (step by step process) and what is the purpose of bacterial culture…

A: Introduction :- Microorganisms can be found in the natural environment, such as soil. They coexist…

Q: E. coli O157:H7 is an organism of concern in contaminated foods. How can the use of MacConkey agar…

A: MacConkeys Agar is a selective and differential culture medium. It specifically select Gram negative…

Q: How can you confirm yeast growing on Sabouraud dextrose agar to be Candida albicans?

A: Introduction : Candida albicans is the most well-known fungus that attacks the human mucosal layer.…

Q: In the bacterial transformation experiment, what is the primary purpose of using an…

A: Bacteria are Prokaryotic microbes which undergo splitting process and form similar cells. Genetic…

Q: Why is aseptic urine collection important when cultures are ordered? If you counted 20 colonies…

A: Urine collection for the urinary tract infection.

Q: Do you think you can also use Invitrogen PureLink Genomic DNA Mini Kit to obtain chromosomal DNA…

A: S. We can also use Invitrogen PureLink Genomic DNA from a pure culture of archaea that is…

Q: The other question is, how many CT value differences is acceptable for duplication?

A: qPCR(quantitative polymerase chain reaction) is a quantitative method of determining the amount of…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.