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Hi, I am working on qPCR quantification for specific bacteria. However, when the CT value achieve greater than 30, it always shown high CT value differences in my duplication no matter I repeat the same set of experiment for those samples with low quality. My question is, what makes this happening? The other question is, how many CT value differences is acceptable for duplication?
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- Horizontal sequence :RIVL Vertical sequence:FMK Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below. NW algorithm. 1. Complete the scoring matrix. Scoring matrix with PAM250 scores: R I V L F M K 2. Set up, initialize and complete the NW matrix. 3. Retrace, align and score alignment(s). Use the arrows and circles for the matrix and path(s). R I V L F M K Align and score all optimal alignments here. PLZ the arrows and circles for the matrix and path(s) AND SHOW ALL possible Alignment Here the following…Horizontal sequence :VIRL Vertical sequence:MKF Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below. NW algorithm. 1. Complete the scoring matrix. Scoring matrix with PAM250 scores: V I R L M K F 2. Set up, initialize and complete the NW matrix. 3. Retrace, align and score alignment(s). Use the arrows and circles for the matrix and path(s). V I R L M K F Align and score all optimal alignments here. PLZ the arrows and circles for the matrix and path(s) AND SHOW ALL possible Alignment32 pls make an intelligen guess because i do not have clear image. please i will upvote do not reject
- * Le TH Sa u GWG AA PE x GWB Bi 31 K S. .com/forms/d/e/1FAlpQLSeRQOhizlj9haFIAG8XZwqYqNjd4lzDAumrUT8-Azr5lcf77g/viewform?hr_ submission=Chkl14ORirMBEhA e A 33rd Period 10th Gr... Maps H Mail - SC458917 -.. A 33rd Period 10th Gr.. E Applied Educationa.. A To-do A researcher uses a logistic model of population growth. Which statement best describes the relationship between time and population size in a logistic growth model? Over time, population size increases without limit. Over time, population size increases and then levels off to a maximum value. Over time, population size decreases and then levels off to a minimum value. Population size is not related to the passage of time. As limiting factors, how do disease differ from a forest fires? thing 1O DELL13 Match each of the QPCR samples (i-v) with the correct amplification plot (A-E) and determine the Ct value for each of the amplification curves. Number of template copies in the QPCR reaction: Amplification plot (A-E): Ct Value 1200 1000 i. 1X 106 ii. 1X 105 iii. 1X 108 iv. 1X 107 V. NTC (No template control) 800 600 400 200 A C 0 5 10 15 20 25 30 Cycles vi. Create a standard curve graph of template copies vs Ct with the data in the table above. Log of Starting Quantity of DNA vrs C+ vii. 26 24 22221 20 18 16 14 12 כי 10 8 6 4 2 0 5 5.5 6 6.5 7 7.5 8 Log Starting Quantity of DNA (Template copies) If a patient sample has a Ct of 20, interpolate the number of template copies at the start of the PCR reaction using the standard curve you created above?why does a GC rich (70-80%) and GC poor (30-40%) give a low read coverage?
- Download BLOSUM30 and BLOSUMB0 substitu- tion matrices and place them side by side on your computer screen. What are the differences between the two matrices? Why do you see these differences?ule_2023_Spring_Stu x M Inbox (1) - Ic21-0506@lclari X =6104_EP#6 - Google Docs ent/d/104sNODGApfrUg768nDS9piPmOB-VzXs86b_t-tDcDF8/edit My Drive - Google... WebAdvisor Main... ols Extensions Zotero Help text T Arial Haplontic life cycle Diplontic life cycle Parthenogenesis Karyotype Histone - 11 + 2 4. Match the words with the correct definitions: | Alternation of generations Job Listing - USF... WT Enrollment Requir... B I U 3 4 X 0 Lecture Video: The Cell Cyc x Chemical Principl... UO UND Orn Z = - 1:3 A type of reproduction that produces a clone of the mother A protein DNA wraps around it to condense the DNA An organism alternates between 1n and 2n with each generation A complete set of chromosomes, as they appear in the nucleus When an organism spends most of its life in the 1n state When an organism spends most of its life in the 2n state 11Which of the ff is incorrect?
- 11:111 11:09 l 5G 4. 64/mydocument IXL - Sign In Pt Dynamic Periodic. E Sign In | MobyM psych textbook Submit Assign Fatal Hazing in College - 12105396 bols Add-ons Help Accessibility Last edit was 9 hours ago -BIUA o0回ロ, 三-- 1 xt Times 12 + ACC 10 11 12 13 14 15 116 171 Anaiysis quesuons 1. Define osmosis. - osmosis is the movement of water from areas of high concentration to low arcas of low concentration 2. What problem did drinking nearly 10 gallons of water create in John's body? An excess amount of water in his bodies, causing cells to take in more water than they are able to. 3. What happened to John's brain cells (hint: connect to tonicity)? m 4. When John made it to the hospital, the doctors gave him a sodium IV solution. Why did the doctors do this? The doctors did this because the water will follow the salt out of the cells. 5. Copy and paste a diagram of a section of the plasma membrane. How is it able to regulate what crosses the membrane? EXTEACELLULAR SPACE (outide of…norypefor he m ch ndohe R le the Problems for practc For each problem state the formula, add the data into the formula, sorve The frst problem has been done for you; this illustrates all the steps you should perform for eacn response pawenA) blirdo 1. Predict the TRC for each of the following individuals. [Write clearyn emuezs blirlo tiertt bas alnensg ari nol 20RT one jerlW () natn Bhe traing noidw 10l noijatoois der oispibni ol 1odmemeA. MALE GENOTYPE FEMALE A of active factor for each TRC= Baseline +( alleles ( active allele actve tactor for eacr TRC= Baseline +(" alleles2 active allele/ AABBCCDE TRC = 80 + (8) (12) TRC = 70 (8) (9) = 80 + 96 176 fingerprint ridges %3D 70 + 72 142 fingerprint ridges %3D = 1. If an A a Bb C cdd male mates with an A a Bb CCDD female, (a) What is the minimum number of ridge-producing genes possible for a child of this couple? (Show your work. Hint: solve gene by gene) (b) what would be the TRC for this child if it is male? (Show your work: give the…QUESTION FROM GENETICS।। THANKS