1 Apply the patient's blood sample to the antibody array. Covalently link antibodies to 2 each glycoprotein to a solid support. 3 Detect fluorescence. 4 Add fluorescently labeled lectins to the array. Each lectin should bind the glycan component of one glycoprotein under investigation.
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Arrange the steps of performing the test to detect the presence of the glycoproteins indicating aggressive cancer in order, from start to finish.
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- Complement activation is a cascade reaction, with each component sequentiallyacting on others, in a similar way to the blood-clotting system. Discuss the effectsthat occur when peptides are generated from the activation by either the classicalor the alternative pathway. This is an immunology questionIdentify three other methods commonly used to visualize lipids on a TLC plate. Specify the type or class of lipids that are detected using these methods.What are the different properties of an antigenEnumerate and discuss the concept of antigenicityIdentify and differentiate the different types of antigenWhat are the different properties of an antibodyDifferentiate the various types of antibodies
- Your Ceftazidime Client has an Infectiun and hes been ordered Im 96h. Auarlable is a vial with 2 5oumg grams of ceftazidime The directions on the via read Ce reconstitute with 250mg 1omis of diluent for a cencertratiun 1 mL. . How much would be lnjected ? cstYou just received the properly labeled blood bank specimen on patient Aran Stark. You decide to collect some background information about her known historical antibodies before beginning the work-up knowing that she has a history of anti-E, anti-K, anti-Jk^a, anti-Fy^a, anti-M and anti-Le^a. Which antibody can be neutralized? Which antibody is destroyed with 0.2M DTT treatment? Which antibody reactivity is enhanced by acidification? Which of the antibodies that are typically IgG in nature are destroyed by enzymes? Which are enhanced by enzymes? Which of these antibodies have been known to cause hemolytic transfusion reaction? Which of these antibodies are known to react at room temperature? Which of these antibodies react best at 37C? * When you complete the work-up, you note that the anti-Jk^a antibody is no longer detectable. Can the patient receive red blood cells that contain the Jk^a antigen? Why or why not?Identify the highlighted part of the antibody O Antigen Binding Site O Constant region O Constant region of a heavy chain O Constant region of a light chain O Disulfide bond O Heavy chain O Light Chain O Variable region O Variable region of a heavy chain O Variable region of a light chain
- A 155-pound patient (height 5'9") is ordered interferon Alfa-2b IM 2 million 3 times a week. The drug is available 10 MU in 1 mL. How many mL will the patient need per week?You just received the properly labeled blood bank specimen on patient Aran Stark. You decide to collect some background information about her known historical antibodies before beginning the work-up knowing that she has a history of anti-E, anti-K, anti-Jk^a, anti-Fy^a, anti-M and anti-Le^a. Which antibody reactivity is enhanced by acidification?Which of the following media is capable of detecting all clinically significant antibodies while avoiding all clinically insignificant antibodies? Question 1 options: Gel technology Tube technique with PEG No single media is capable of doing this Solid phase
- A technologist failed to notice that the centrifuge had not properly centrifuged the test tubes prepared for antibody identification. The time of centrifugation was 15 seconds instead of 30 seconds. What would be the potential error in the interpretation of this test? can please any one provide me ans?The technician decided to antigen type the patient to confirm predictions from the antibody panel. Antigen type results are given below. Given these results, which of the following statements is BEST supported? Rh Kell Duffy Kidd anti-D anti-C anti-E anti-c anti-e anti-K anti-Fya anti-Fyb anti-Jka anti-Jkb 3+ 0 3+ 3+ 0 0 0 3+ 2+ 2+ Question 7 options: A) K and Fya antibodies are likely because patient would not readily produce antibodies against their own cells. B) An anti-e antibody must be present due to the lack of antigen on the patient cells. C) Jka and Jkb antibodies are likely because an autoantibody is present. D) Rh antibodies are likely because the patient is Rh negative.Which of the following statements is true? O Streptolysin-O is a growth factor for white blood cells. O Anti-streptolysin-O antibodies peak several months after initial symptoms. O Currently, the main method for detecting anti-streptoryan-G antibodies in radal immansattfo O Streptolysin-O does not induce an immune response. In the classical method of testing for anti-streptolysin-O antibodies, a positive reaction is indica intact red blood cells