A technologist failed to notice that the centrifuge had not properly centrifuged the test tubes prepared for antibody identification. The time of centrifugation was 15 seconds instead of 30 seconds. What would be the potential error in the interpretation of this test?
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A technologist failed to notice that the centrifuge had not properly centrifuged the test tubes prepared for antibody identification. The time of centrifugation was 15 seconds instead of 30 seconds. What would be the potential error in the interpretation of this test? can please any one provide me ans?
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- https://youtu.be/w7aIxiZQ60g Multiplexing agglutination https://youtu.be/uWStmyJ5Qc0 This is the multiplexing agglutination. Lab report I don’t really know what to talk about, the data, conclusions and the purpose of this. Need help pleaseYou just received the properly labeled blood bank specimen on patient Aran Stark. You decide to collect some background information about her known historical antibodies before beginning the work-up knowing that she has a history of anti-E, anti-K, anti-Jk^a, anti-Fy^a, anti-M and anti-Le^a. When you complete the work-up, you note that the anti-Jk^a antibody is no longer detectable. Can the patient receive red blood cells that contain the Jk^a antigen? Why or why not?The technician decided to antigen type the patient to confirm predictions from the antibody panel. Antigen type results are given below. Given these results, which of the following statements is BEST supported? Rh Kell Duffy Kidd anti-D anti-C anti-E anti-c anti-e anti-K anti-Fya anti-Fyb anti-Jka anti-Jkb 3+ 0 3+ 3+ 0 0 0 3+ 2+ 2+ Question 7 options: A) K and Fya antibodies are likely because patient would not readily produce antibodies against their own cells. B) An anti-e antibody must be present due to the lack of antigen on the patient cells. C) Jka and Jkb antibodies are likely because an autoantibody is present. D) Rh antibodies are likely because the patient is Rh negative.
- What is an antigen? What is an antibody? Why does each test include a control line? What specimen would be collected for an antigen test? Why is it important to not touch the tip of the swab?What specimen would be collected for an antibody test? How many lines for a positive antigen test?How many lines for a negative antigen test? What do three lines show in an antibody test?What are the important considerations that you have to remember in antibody screening? What are the important considerations that you have to remember in antibody identification What is the role of antibody screening in pretransfusion compatibility testing?The secondary antibodies used in the ELISA experiment are conjugated (attached) to what enzyme? PRP HRP Rh АВО
- Can you please check and answer the questions that not answered, that is not graded https://www.studocu.com/en-us/ document/university-of-arkansas/microbiology/other/immunology-lab-worksheet-student/7960193/view Questions from ELISA Simulation Introduction Document (posted in Lab Module 8 folder): 1. What term is the word “antigen” derived from? 2. Where are antibodies found in the body? Plasma of the blood 3. In general, what is the cause of an autoimmune disease? 4. What kind of antibodies are present in patients with systemic lupus erythematosus (SLE)? 5. What does ELISA stand for? enzyme-linked immunosorbent assay 6. In general, what is an ELISA test used to detect? to measure the concentration of antibodies or antigens 7. In an indirect ELISA, which antibody is linked to the enzyme? (i.e the primary antibody from the blood serum or the secondary anti-human antibody) Questions from HHMI Virtual Immunology Lab Website Diagnosis…Why does the antibody titer determination use twofold dilutions ofthe antiserum rather than 10-fold dilutions?You just received the properly labeled blood bank specimen on patient Aran Stark. You decide to collect some background information about her known historical antibodies before beginning the work-up knowing that she has a history of anti-E, anti-K, anti-Jk^a, anti-Fy^a, anti-M and anti-Le^a. Which antibody is destroyed with 0.2M DTT treatment?
- You just received the properly labeled blood bank specimen on patient Aran Stark. You decide to collect some background information about her known historical antibodies before beginning the work-up knowing that she has a history of anti-E, anti-K, anti-Jk^a, anti-Fy^a, anti-M and anti-Le^a. Which antibody can be neutralized? Which antibody is destroyed with 0.2M DTT treatment? Which antibody reactivity is enhanced by acidification? Which of the antibodies that are typically IgG in nature are destroyed by enzymes? Which are enhanced by enzymes? Which of these antibodies have been known to cause hemolytic transfusion reaction? Which of these antibodies are known to react at room temperature? Which of these antibodies react best at 37C? * When you complete the work-up, you note that the anti-Jk^a antibody is no longer detectable. Can the patient receive red blood cells that contain the Jk^a antigen? Why or why not?Wil completed a full plate titer assay and there were 25 plaques on his 10^-7 plate. What is the titer of his high titer lysate? 10uL of each dilution were used for the plaque assays. O 2.5X10^11 pfu/mL O 2.5X10^7pfu/mL O 2.5X10^9 pfu/mL O 2.5X10^8 pfu/mL O 2.5X10^10 pfu/mLWhich portion of the blood is used for the Elisa test? Explain.