Bio 370 Paper 3 Worksheet

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Arizona State University *

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370

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Biology

Date

Dec 6, 2023

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docx

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2

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Biology 370 Principles of Muscle Physiology & Energetics Paper Discussion 3 Worksheet Participant Names (max 5): Brand MD, Turner N, Ocloo A, Else PL & Hulbert AJ. 2003. Proton conductance and fatty acyl composition of liver mitochondria correlates with body mass in birds. Biochemistry Journal . 376: 741-748. In preparation for class, please read the above referenced paper, which can be found in the Module 10 on Canvas. Due Date: completed worksheet is due on November 16 th , 2023 at 8 p.m. on Canvas Question 1. (3 marks) a) Provide one reason why mitochondrial proton leak is potentially detrimental to metabolism. Explain why it is detrimental. (1 mark) b) All mitochondria show some degree of proton leak, suggesting that it may have a physiological purpose. Propose one potential physiological benefit to mitochondrial proton leak and explain why it would be beneficial. (1 mark) c) Why do you think the mitochondria from ectotherms generally have low proton leak? (1 mark) Question 2. (3 marks) This paper states a goal for the study in the last paragraph of the introduction, but the authors did not frame their investigation around a clearly articulated hypothesis and they didn’t provide specific predictions for their study. Please provide a posteriori (after the fact) hypothesis for this study and at least two predictions. “In the present study, we analysed the relationship between phospholipid fatty acyl composition of liver mitochondria , mitochondrial proton conductance and body mass in birds to investigate whether the allometric relationships found in mammals are general for all endotherms, or specifically mammalian.” Hypotheses (1 mark) : 1
Predictions (2 marks) : Question 3. (2 marks) Oligomycin is a selective inhibitor of the F 1 F O -ATP synthase (aka complex V of the electron transport system) and is used to help address the goals of their study. How does the use of oligomycin help the authors address their study goals? (1 mark) What unique data are generated through the use of this drug that are important to addressing the study objectives. (1 mark) Question 4. (2 marks) Figure 2 shows the ratios of different mitochondrial respiration rates (respiratory control ratios) relative to the body mass of the birds under investigation. What do the data presented in Figure 2 reveal about the relationship between mitochondrial function and body mass ? Question 5. (2 marks) The mitochondrial respiration data presented along the y-axis of Figure 4 (a and b) is presented at a constant membrane potential (170 mV) across the inner mitochondrial membrane. Why is it important to do this in the context of assessing proton leak. Question 6. (2 marks) Briefly describe using non-expert language how the data presented in Figure 5 relates to, and possibly explains, the data presented in Figure 4b. Total: /14 2
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