Sene edits can be made to Eukaryotic DNA by using a complex composed O Progenitor cell and 4 master regulatory genes O Cyclic AMP and CRP protein Ubiquitin protein and CRISPR O Guide RNA and Cas 9 protein O Histone proteins and methyl groups

Q: A. The double-stranded DNA sequence for a bacteria is shown below and it's coding for a hypothetical…

A:

Q: Short tandem repeats (STR) profiling is based on O A. the fact that many foods are being genetically…

A: The method of DNA profiling used today is based on PCR and uses simple sequences or short tandem…

Q: Which of the following regions of the DNA is NOT transcribed by RNA polymerase? 1. -10/-35 promoter…

A: RNA polymerase is an enzyme that helps in the synthesis of RNA from the DNA template. This enzyme…

Q: you carry out DNA editing using CRISPR whter the editing template DNA has strands labeled with heavy…

A: DNA (Deoxyribonucleic acid) is edited with the help of several molecular tools. CRISPR (Clustered…

Q: an opening reading frame is defines by _ 1 absence of highly repetitive sequence 2homology with…

A: To locate the gene sequence inspection are used because the gene has distinctive features which…

Q: The double-stranded DNA sequence for a bacteria is shown below and it's coding for a hypothetical…

A: The central dogma of life involves DNA replication, transcription, and translation into proteins.…

Q: In biotechnology, an organism modified by gene splicing method is called Which of the following…

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Q: Match the term with its description.

A: There are various enzymes and gene components that are studied in techniques of genetic engineering.…

Q: To quantify the transcription product of a specific gene in E. coli, you may use (select all correct…

A: Introduction The Process Of Transcribing A Piece Of DNA Into RNA Is Known As Transcription.…

Q: Page 6 of 6 Describe the cloning vectors that would be used to clone each of the following DNA…

A: A cloning vector is a short fragment of DNA that allows the incorporation of foreign DNA for…

Q: The gene for the primase enzyme is mutated and is nonfunctional. What is the result of such a…

A: DNA replication unlike transcription cannot start ab initio and requires a primer that is elongated…

Q: What does Cas9 do in CRISPR-Cas9 editing? O It makes double stranded breaks at specific locations in…

A: According to guidelines we have to answer the first question only. so please kindly post the…

Q: Real-time PCR is used in many transcription? Select one or more: O a. Gene transcription qu b. Ob.…

A: Note- According to the guidelines only one question can be answered at a time. Hence, the first…

Q: Examine the DNA fragment sequence below. Your job is to design primers for PCR that would be able to…

A: Answer: REPLICATION of DNA : It is the process in molecular biology where  DNA strands are used to…

Q: In prokaryotic organisms transcription is initiated: O downstream from a Shine-Delgarno sequence a…

A: Option C

Q: This sequence element is found in RNA and is critical for prokaryotic translation. O Shine-Delgarno…

A: Translation involves translating the sequence of a messenger RNA (mRNA) molecule to a sequence of…

Q: The gene for the primase enzyme is mutated and is nonfunctional. What is the result of such a…

A: The process associated with the duplication of genetic material or DNA can be described as…

Q: I. List the sequences of RNA that would be transcribed from the following DNA template sequences. а.…

A: Transcription is the initial stage in gene expression, when genetic information is utilised to build…

Q: Oligonucleotide-directed mutagenesis must be used instead of site-directed mutagenesis to create a…

A: DNA sequence is not a static entity. It can be subject to various heritable genetic changes. A…

Q: The enzymatic activity necessary for proofreading is: O reverse transcription O ligase Oendonuclease…

A: DNA proofreading is the process through which the identification and the correction of errors in the…

Q: The recognition site for Spel is A'CTAGT. Which of the following restriction sites when digested…

A: Restriction site - Each restriction enzyme identify specific sequence of nucleotide, known as…

Q: Great! Now you've got the Wooly Mammoth hemoglobin gene sequence with just the exons! You generate a…

A: Restriction enzymes are a specific group of enzymes that can recognize a specific short sequence of…

Q: the target DNA

A: CRISPR: It stands for Clustered Regularly Interspaced Short Palindromic Repeats. These are the…

Q: Which of the following molecules|are involved in gene expression? Choose all that apply. O RNA…

A: Nucleic acid such as DNA is the major carrier for genetic information in almost all living…

Q: The LEAST critical part of a plasmid needed to create a transgenic cow that expresses a foreign…

A: Transgenic cows are produced by DNA cloning methods in which a foreign gene is inserted into the…

Q: During the differential sene expression module.which of the following was used as template in the…

A: Differential gene expression is the process through which cells differ from one another because of…

Q: Which of the following is TRUE regarding reading frame? O a) An open reading frame can have many…

A: Answer: Introduction: A reading frame is an order of nucleotide triplets which signifies amino…

Q: * 00 T 品 11. Protein expression can be regulated by RNA interference (RNAI) . Which proteins and…

A: RNA interference is a biological method which is used for silencing gene expression. It involves…

Q: Following is the nucleotide sequence in a segment of a strand of DNA 5'- AATTGGCTCTATAAT-3' Give the…

A: Adenine, thymine, guanine, and cytosine are the bases of the DNA. The mRNA is a single-stranded…

Q: DNA sequencing can help us to identify mutations withingenes. The following data are derived from an…

A: Any change in the sequence of DNA is termed as mutation, it can be due to physical or chemical…

Q: A. The double-stranded DNA sequence for a bacteria is shown below and it's coding for a hypothetical…

A: Transcription is the process where mRNA will from form DNA, then mRNA will from protein in…

Q: Cas9 endonuclease can be targeted to specific sequences in the genome using a OA. PAM site O B.…

A: Answer:- Option B is the correct choice. Cas9 endonuclease is a bacterial RNA-guided substance   It…

Q: Expression of a cloned eukaryotic gene in a bacterial cellinvolves many challenges. The use of mRNA…

A: Genetic engineering involves the process of using recombinant DNA (rDNA) technology to alter the…

Q: CRISPR in nature evolved as a "bacterial immune system" or "genetic vaccine" to target infecting…

A: CRISPR stands for "Clusters of Regularly Interspaced Short Palindromic Repeats". Cas9 is the…

Q: Which of the following would not be used when trying to express a eukaryotic gene in a prokaryote? O…

A: Eukaryotes and prokaryotes are the two types of cells, wherein the prokaryotes are the primitive…

Q: Synthesis of hybrid genes can occu Directed mutagenesis O Using overlap primers O RT-PCR

A: PCR technique of amplification of a given molecule of DNA using taq polymerase enzyme.

Q: To quantify the transcription product of a specific gene in E. coli, you may use (select all correct…

A: Transcription  It is a biological processe where RNA is made by using DNA as a template.

Q: You have completed an in vitro mutagenesis experiment to create an G to Tmutation in the coding…

A: After first being developed by Frederick Sanger and colleagues in 1977, Sanger sequencing became the…

Q: Frameshift mutations can be caused by: strand slippage. O deletions. depurinations. intercalating…

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Q: A mistake during DNA replication leads to a mutation in the nucleotide sequence shown below. DNA DNA…

A: Mutations are a genetic phenomenon in which alterations in the sequence of nucleotides of DNA occur.…

Q: Frameshift Insertion Mutation – Can be one or more nucleotides. 5’   ATG   GGG    AAA    GAT    TAT…

A: Any heritable change in an individual's genetic makeup is referred to as a mutation. It's when a…

Q: The double-stranded ONA sequence for a bacteria is shown below and it's coding for a hypothetical…

A: The antisense strand of the DNA sequences acts as a template for the synthesis of mRNA molecule by…

Q: I. The DNA template, 3'–CGTTACCCGAGCOCGTACGATTAGG–5', was exposed to a mutagen, resulting in the…

A: Hi, Since you have posted a question with multiple sub-parts, we are answering only first one for…

Q: For the ovalbumin gene shown in Figure 14.3, where would the 5′ untranslated region and 3′…

A: Mature mRNA is an mRNA that is modified post-transcriptionally. Mature mRNA carries information from…

Q: The following is an example of what type of mutation? Normal DNA: 5' - ATGACGTCA - 3' Mutated DNA:…

A: Thank you for the question  Answer = The correct answer is option A (Deletion). Explanation :- This…

Q: In the CRISPR čas9 system, at which stage of the system the target gehe double strand breaks? O a.…

A: Since you have posted a question with multiple parts, we will solve the first parts for you. To get…

Q: Vhich of the following are required to set up a CRISPR-Cas9 experiment to fix a mutation in a gene…

A: CRISPR or Clustered regularly interspaced short palindromic repeats is a gene editing technique in…

Q: CRISPR/Cas9-based gene editing in genetic studies have the following attractive feature(s) in…

A: CRISPR (Clustered Regularly Interspaced Short Palindomic Repeats) is a group of DNA sequences…

Q: EboV RNA from Guinea Pig EboV RNA from Guinea Pig GAU ACG UUC GUC AAU EboV DNA CTA TGC AAG CAG TTA…

A: Substitution mutation- substitution of a base pair for another during DNA replication is the most…