Short tandem repeats (STR) profiling is based on O A. the fact that many foods are being genetically modified and this test allows food health officials to identify the transgenic ones. OB. the fact that you can clone mammals through fusion of one somatic (non-sex) cell with an egg cell whose nucleus has been removed O C. the fact that one strand of DNA can be turned into millions of identical copies by a process that heats and cools DNA and builds it using DNA polymerase and primers. the fact that people's DNA is filled with short sequences, like "TCAT" that are found in different numbers in each person. OD

Short tandem repeats (STR) profiling is based on O A. the fact that many foods are being genetically modified and this test allows food health officials to identify the transgenic ones. OB. the fact that you can clone mammals through fusion of one somatic (non-sex) cell with an egg cell whose nucleus has been removed O C. the fact that one strand of DNA can be turned into millions of identical copies by a process that heats and cools DNA and builds it using DNA polymerase and primers. the fact that people's DNA is filled with short sequences, like "TCAT" that are found in different numbers in each person. OD

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 1TYK

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When molecular biologists carry out a PCR, they have a range of different DNA polymerase enzymes on the market that they can purchase, some of them complete proteins taken from different species, and some modified proteins. A molecular biologist plans to buy a Taq DNA polymerase to PCR a DNA fragment for cloning into an expression vector. e a. Which two polymerases are used during DNA replication in the cell? e b. List the different enzymatic properties of these DNA polymerases and explain the e specific situations they would be used during DNA replication. e c. Explain which enzymatic properties a molecular biologist might want in a Taq e polymerase used for the experiment described above.
PCR is used for cloning DNA and after about 30 cycles, the number of copies DNA will have increased about a billion times. Why is the DNA polymerase from the bacteria Thermus.aquaticus used in PCR? Because it uses primers specific to sequences in a disease causing gene or organism to diagnosis diseases, etc. O It is used to flank the gene to be copied. O It allows scientists to be able to determine the variable number of tandem repeats of minisatellites or microsatellites to determine an individual's DNA fingerprint. O It is present and needed because the enzyme is resistant to very high temperatures since the bacteria live in hot springs. Due to the walls of the he plant cell, which are thick; therefore it is used to degrade the walls for PCR to begin.
O e. Gene amplification CLEAR MY CHOICE To study the function of any gene of interest you would perform the loss and gain of function approaches by either deleting or re-expressing the gene of interest, which of the following can be used to determine and quantify the activity of the gene? Oa. Western blotting O b. Gene knockdown O. PCR/OLA O d. Microscopy O e. DNA hybridization Paternity testing can be detected most precisely by using technique
Which of the following steps is NOT involved in gene cloning? Lütfen birini seçin: O a. Transferring recombinant vector into the host cell O b. Insertion of a gene into a vector O c. Selection of cells with recombinant vectors O d. Removing nucleus from donor cell O e. Isolation of a gene to be cloned
OO HUAWEI Nova 2 Plus DUAL CAMERA tem (Academic) c. Heat shock. d. Floral dip. O e. Electrophoresis. The hybridization between DNA during the library screening process take place between probs and CDNA of the organism when: Select one: O a. Van der Waals forces. O b. Hydrogen bonds form between the complementary DNA sequences. O c. Covalent bond form between the complementary the DNA sequences. O d. Glycosidic bonds. e. Phosphodiester bond and cause ligation the DNA sequences. Next
About transcriptomics and genomics, answer: 4. Can we replicate RNA? How can we use PCR and Sanger sequencing to study the ranscriptome? a. b. How will the genome of two different cells of the same animal compare? How will the transcriptome oftwo different cells of the animal compare? c.
The selection of the clones in plasmid-based directed mutagenesis depends on a. Replication error during the PCR O b. Reversing the resistance gene status O C. Blue colonies with disrupted LacZ O d. White colonies with disrupted LacZ O e. Plaques formation To overexpress a known gene of interest to produce high quantity of an industrial product you need to us a. Anti-sense RNA Ob. Anti-sense oligonucleotide O C. Ribozymes cloning Od. RNAi O e. Plasmid based directed mutagenesis. The CRISPR RNA in type II is composed of a. RNA sequence complementary to spacer DNA and CRISPR Ob. CRISPR RNA loop and spacer DNA O C. RNA sequence complementary to CRISPR DNA and spacer DNA O d. PAM sequence and Tracer RNA O e. Tracer RNA complementary to CRISPR RNA and the viral RNA The replication errors can be caused due to
DNA molecular with complementary sticky ends associate by (a) covalent bonds (b) hydrogen bonds (c) ionic bonds (d) disulfide bonds (e) phosphodiester linkages
Match each term with the most suitable description. ___ DNA profile a. GMO with a foreign gene ___ Ti plasmid b. alleles commonly contain them ___ cDNA synthesis c. a persons unique collection of short tandem repeats ___ SNP d. requires reverse transcriptase ___ transgenic e. cuts DNA ___ restriction enzyme f. used in plant gene transfers
Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymes
PCR can be used ______. a. as a cloning vector b. in DNA profiling c. to modify a human genome
Cloning Genes Is a Multistep Process The following DNA sequence contains a six-base sequence that is a recognition and cutting site for a restriction enzyme. What is this sequence? Which enzyme will cut this sequence? (See Figure 13.5 for help.) 5 CCGAGGAAGCTTAC 3 3 GGCTCCTTCGAATG 5