II. Lipids from an organic sample are extracted separately by column chromatography using the ff eluants: 1st eluant chloroform: methanol: water (6:3:1) 2nd eluant-methanol: chloroform (9:1) 3rd eluant - petroleum ether: ethyl ether (1:1) Test results for each eluate are tabulated below. Identify the possible lipid present in each eluate. Test Reagents 1. KHSO4 2. HNO3,(NH4)3M004 3. CHCl3, acetic anhydride, conc. H₂SO4 4. a-naphthol, conc. H₂SO4 5. Hydroxylamine HCI, FeCl3 6. ninhydrin reagent POSSIBLE LIPIDS: Triacylglycerol, cholesterol, sphingomyelin, lecithin 1st eluate + + + + 2nd eluate + 3rd eluate + +
II. Lipids from an organic sample are extracted separately by column chromatography using the ff eluants:
1st eluant - chloroform: methanol: water (6:3:1) 2nd eluant - methanol: chloroform (9:1)
3rd eluant – petroleum ether: ethyl ether (1:1)
Test results for each eluate are tabulated below. Identify the possible lipid present in each eluate.
Based on the results above,
_________________ 1. _________________ 2. _________________ 3.
_________________ 4.
_________________ 5.
answer the following questions:
What visible positive result for test reagent no. 5?
Give the name of chemical characterization test for test reagent no. 3?
What class of lipids will give a visible positive result on the test mentioned in question no. 2?
Give the chemical name of the product formed after giving a positive result for test reagent no. 1?
Test reagent no. 1 is used to test for what organic compound?
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