How do you do a DNase footprint assay. What did it demonstrate in regards to the UP elements binding site? 8022 -2NA 8022 ANK -31 INAP
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What did it demonstrate in regards to the UP elements binding site?
by Bartleby Expert- These are the results after running Agarose Gel Electrophoresis of the cut/uncut pUC19 plasmid and isolated Genomic DNA at 120 V for 30 minutes. Can you explain what this gel means?During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricityWhat assay uses the fact that activators can be separated into DNA binding and activating domains? What information do you gain from this assay? Describe briefly how to perform this assay.
- A cloning vector map is shown below. EcoRI Bam Ban Hind P-galactosidase Amp Bam Bam EcoRI Ori C Which restriction site is best for inserting a DNA fragment for selection of chimeric plasmid containing colonies? 1) They're all equally good. 2) Hindll 3) EcoRI 4) BamHIWhich of the DNAs shown in Figure would move fastest during agarose gel electrophoresis?Draw a gel to represent the band shift assay result for testing the following mixtures: (1) Eukaryotic DNA along(2) Eukaryotic DNA + TFs(3) Eukaryotic DNA + RNA polymerase II(4) Eukaryotic DNA + TFs + RNA polymerase II(5) Eukaryotic DNA + TFs + RNA polymerase II + nucleotidesBands for smaller molecules can be ignored
- E. coli K12 genomic DNA (expected PCR product was 3075bp). For expression the gene was cloned into the expression plasmid pET28a (Novagen), where expression is under control of the inducible T7 promoter.Give information about the enzyme being purified, its expression in E. coli and the property being used for its purification. please provide the references for the answer.A recombinant protein corresponding to 52 repeats of the peptide sequence YSPTSPS was covalently linked to agarose beads to make an affinity column. The beads were incubated with a nuclear extract and the protein:beads became phosphorylated. More nuclear extract was run over the column repeatedly and then the beads were washed extensively. The bound proteins were eluted from the column and when they were separated on a SDS-PAGE gel many bands appeared. What cellular process(es) might these proteins be involved in? Options: splicing polyadenylation DNA replication a and b only a, b and cPlasmid DNA Extraction by Alkaline Lysis Why is Glacial acetic acid added to solution? What is the function of Potassium acetate in this solution?
- What volume of 4X loading buffer must be added to 21 micro L of DNA in a technique for DNA sample preparation for agarose gel electrophoresis to generate a 1X buffer solution?Map of pUC18 is shown on the here. Describe how to select recombinant clones if a foreign DNA is inserted in to the polylinker site of pUC18 and then introduced into E. coli cells.. Hindll Sphl Sbfl Pstl BspMI Acci Hincli Sall Xbal BamHI Aval Smal Xmal Acc651 Kpnl Banll Eco53kl Sacl Apol ECORI lacz MCS LacR binding site Plac PUC18 Amp 2686 bps PMB1 oriwhat type of gel(in terms of material) can be more suitable for the electrophoresis of 500-1000bp DNA fragment and why?
