Concept explainers
Interpretation:
The given table should be completed.
Concept introduction:
The formula to calculate specific activity of the protein is as follows:
The formula to calculate the percentage yield is as follows:
The formula to calculate the purification level is as follows:
Answer to Problem 18P
The given table is completed as follow:
Explanation of Solution
The formula to calculate specific activity of the protein is as follows:
Specific activity for crude extract is as follows:
Substitute
Specific activity for
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Specific activity for DEAE-cellulose chromatography is as follows:
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Specific activity for gel-filtration chromatography is as follows:
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Specific activity for affinity chromatography is as follows:
Substitute
The formula to calculate purification level is as follows:
Purification level for crude extract:
Substitute
Purification level for
Substitute
Purification level for DEAE-cellulose chromatography:
Substitute
Purification level for gel-filtration chromatography:
Substitute
Purification level for affinity chromatography:
Substitute
The formula to calculate percentage yield is as follows:
Percentage yield for crude extract:
Substitute
Percentage yield for
Substitute
Percentage yield for DEAE-cellulose chromatography:
Substitute
Percentage yield for gel-filtration chromatography:
Substitute
Percentage yield for affinity chromatography:
Substitute
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Chapter 3 Solutions
Biochemistry
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