If 4 mL of 1 M NaOH is added to 100 mL buffer, would it still be a usable buffer according to the conventions? Explain why or why not.
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If 4 mL of 1 M NaOH is added to 100 mL buffer, would it still be a usable buffer according to the conventions? Explain why or why not.
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- 50X TBE is 87 mM Tris-borate and 4 mM EDTA at pH 8.3. What is the molarity of each of the chemicals in 1X TBE buffer?A 500 mL sample of a 0.100 M formate buffer, pH 3.75, is treated with 5 mL of 1.00 M KOH. What is the pH following this addition?Draw titration curve for compound X when NaOH equivalence(s) is/are added. Indicate the buffering region(s) with a box.
- What would be the working stock concentration (in molarity) if you diluted 250 uL of a stock concentration of 10 uM in a total volume of 1 mL?You need to prepare an acetate buffer of pH 5.43 from a 0.621 M acetic acid solution and a 2.95 M KOH solution. If you have 730 mL of the acetic acid solution, how many milliliters of the KOH solution do you need to add to make a buffer of pH 5.43? The pKa of acetic acid is 4.76. Be sure to use appropriate significant figures.I want to make a buffer solution containing 1 M glycene pH = 10 (Na+), 1 mM ZnCl2 , 1 mM MgCl2. I have the stock solutions 1 M glycene, 1 M MgCl2, 1 M ZnCl2, and 10 M NaOH. My final volume of buffer solution needs to be 250ml. how much (in ml) of each solution should i put into my buffer?
- You need to prepare 1L of a 5×TE stock solution (1×TE buffer:10mM Tris,1mM EDTA).How much of Tris you need? How many ml of the 0.5 EDTA stock solution do you need?What is the pH of the buffer 0.17 M Na2HPO4/0.25 M KH2PO4?How much of the enzyme proteinase k (solute) is required to make 250ml of a solution with a concentration of .01mg/ml? (Weight/volume ratio)