Begining with 1 M concentrations of each reactant and product at pH=7 and 25.0 degrees C, calculate the K'eq of the reaction Pyruvate + NADH <=> Lactate + NADH+H+. Note the temperature of this reaction will not affect the standard reducton potential delta E'o in the table 13-7b.
Q: III. Effect of inhibitor Color of bromothymol blue indicator Test Tube Original color Final color A…
A: Bromothymol blue is a pH indicator that commonly turns yellow in acidic solution and turns blue in…
Q: Which of the following statements regarding size exclusion chromatography is false? During…
A: Hi! Thank you for the question, as per the honor code, we are allowed to answer the first…
Q: What are the biomolecules that are used to biosynthesize a purine ring in the de novo pathway –…
A: Purine de novo synthesis is a time-consuming process. It happens actively in the liver cytosol,…
Q: 2. 1 Calculate the overall charge (pH 7) on the following three polypeptides and answer the…
A: Hi! Thanks for your question. As you have posted multiple questions and have not mentioned which…
Q: Colchicine is used to treat gout. It alters cytoskeleton function. Why do you think it is useful for…
A: Gout is a frequent and complicated form of arthritis that can strike anyone at any time. It's…
Q: A. Name the two proteins that induce a negative feedback in this pathway and explain why both…
A: Since, you have posted multiple questions we will solve the first question for you. If you want any…
Q: You'lre hungoy, body Cmuttiple g the following e optim posei ble) which Occur in Our toonelete (A) À…
A: Hungryness is a feeling that stimulates food intake. There are number of pathways that stimulate…
Q: In cation exchange chromatography,
A: Any positively charged ions that come into contact with the resin will be exchanged with other…
Q: 3. The trp repressor is a homodimer with 107 amino acid residues per monomer. The content of proton…
A: As content of proton dissociable group presented in table, here we can count the charge status of…
Q: ection steps! Which of the following are proper disir ORemove organic matter O Disinfect only O…
A: Microscopic organisms such as bacteria, fungi (mold and yeast), protists, archaea, algae,…
Q: Identify if the following is a pyrimidine/purine nucleotide or a pyrimidine/purine nucleoside and…
A: The nucleic acids are known as polynucleotides. Monomeric units of nucleic acids are called…
Q: Explanations are not needed. Direct answers are enough. II. True or False a. An effort is…
A: The protein molecules after their folding have exposed patches of charged amino acids on their…
Q: Eukaryotic polymerases have the same number of subunits as prokaryotic ones. True False
A: The process of creating an RNA copy of a gene sequence is known as transcription. The copy is known…
Q: What are nucleosides?
A: Nucleosides are compounds consisting of purine or pyrimidine bases linked to sugar. The nucleobase…
Q: 2. Calculate the overall charge (pH 7) on the following three polypeptides and answer the questions…
A: Hi! Thank you for the question. We are authorized to answer three subparts at a time, since you have…
Q: *Which of the following statements about allosteric enzymes is NOT true? Question 10 options: - They…
A: Enzymes are biocatalysts that increase the rate of a reaction by a factor of several. Living cells…
Q: There are two types of nucleic acids-DNA and RNA, both are composed of nucleotide subunits. Complete…
A: DNA and RNA are nucleic acids composed of nucleotide units. Each nucleotide is composed of a pentose…
Q: You are analyzing the peptide ala-ile-glu-lys-phe-val- tyr-cys. If you treat the peptide with…
A: Proteins or peptides are a linear chain of amino acids attached together via peptide bonds. Natural…
Q: reaction
A: Hess law is mainly used to find out specific unknown enthalpy change. It is particularly useful for…
Q: Choose among proteins A, B, C, and D. 1. Protein that will most strongly bind to an anion exchange…
A: Chromatographic techniques are used to separate specific molecules from a mixture of different…
Q: Derive the amino acid sequence that is coded for by each of the following mRNA sequence. 5' CAA…
A: Amino acid are molecules which are combine to form a protein molecules.Amino acid are synthesized by…
Q: a. What is isoelectric point? (Round your answer to two decimal places, for example: 0.13 or 1.45 or…
A: The isoelectric point (pI) is the pH at which a particular molecule carries no net electrical…
Q: Which of the following statements is TRUE for nonpolar amino acid residues of polypeptides or…
A: They are hydrophilic and found buried within proteins FALSE. Water soluble proteins contain…
Q: 2. Glvcolate is oxidized into Glvoxylate by cytochrome Cusing the glycolate oxidase enzyme, The two…
A: Glycolate oxidase is a key enzyme involved in the conversion of glycolate to glyoxylate during…
Q: Which class of proteins is more easily denatured—fibrous or globular?
A: In an amino acid-chain molecule, protein structure refers to the three-dimensional arrangement of…
Q: Please name the missing product marked x in this reaction H-Q АТР + C--0 - X + ADP
A: In such reaction ATP molecules is reacts with carbonate molecule which gives ADP and carboxyl…
Q: estion properly and accordingl
A: Saponification is the process of forming a metallic salt of a fatty acid, which is referred to as a…
Q: Give 5 examples of molybdenum complexes. State their physical and chemical properties and their…
A: The atomic number of molybdenum is 42 and it is represented by the symbol 'Mo'. On the earth,…
Q: ng glycolysis, t
A: Disclaimer: “Since you have asked multiple question, we will solve the first question for you. If…
Q: Calculate the actual free energy of hydrolysis of ATP, delta Gp in the erythrocytes of a new…
A: Actual free energy (∆G) is the maximum amount of energy which is available to perform work. Standard…
Q: Calculate the values of the
A: Enzyme kinetics is a study of the rate of enzyme catalyzed reactions. In Michaelis-Menten kinetics,…
Q: A polypeptide with a net positive charge at physiologic pH (~7.4) most likely contains amino acids…
A: are non-polar. Doesn't contribute to charge o the polypeptide.
Q: Describe the ion dynamics of the muscle-contraction process.
A: Tension-generating regions within muscle cells are activated during muscular contraction. Muscular…
Q: Given Poly-L-Lysine and Epsilon Poly-L-Lysine, which polymer(s) can easily dissolve in a saline…
A: Poly-L-Lysine are several types of lysine as homopolymers.Poly-Lysine enhances electrostatic…
Q: The herbicide glyphosate (Roundup®) kills plants. Discuss all the Biochemistry involved as to why…
A: Herbicides are the chemicals used to kill unwanted species of plants growing around the…
Q: 6. DNA electrophoresis uses polyacrylamide gel for separation. a) True b) False 7. Agarose is a…
A: DNA is composed of nucleotides attached via phosphodiester bonds. DNA act as genetic material in…
Q: Among these amino acid combinations listed above, only the combination of Lys and Glu have side…
A: The tertiary structure of proteins are stabilized by non covalent interactions like hydrogen…
Q: Name the following nucleotide: HN P-0-P-0-CH2 OH
A: Nucleic acids are macromolecules. These are of two types - Deoxyribonucleic acid (DNA) and…
Q: Why does it make sense that under conditions of low ATP levels in the cell the pyruvate carboxylase…
A: Glucose is converted to pyruvate through the process of glycolysis in the cytoplasm. The process of…
Q: king of acid
A: Acid is the substance which turns blue litmus to red. Acid contains more H+ ions. Acids acts as…
Q: 20. Beta-oxidation of fatty acids takes place at A. peroxisome B. mitochondria C. mitochondria and…
A: Beta oxidation of fatty acid - Process via which the fatty acids get broken down for energy…
Q: Which base compositions for single-stranded RNA are possible? %A %G %C %T %U OA. All of the answers…
A: RNA are single stranded, their nucleobases donot participate in base pairing. According to the…
Q: A pyrimidine(s) with a H-bond acceptor its middle position is/are a Cytosine b Uracil c…
A: Hydrogen bond donor is the atom that do not provide a hydrogen atom for hydrogen bonding. They…
Q: ACTIVITY 7.2.2 Show and explain how exactly the condensation reaction to form a nucleotide happens.…
A: Nucleotides are the phosphoric acid esters of nucleosides with the phosphate group. Nucleotides are…
Q: Which of the following condition is NOT associated with riboflavin deficiency? * (Please choose one…
A: Riboflavin is a water soluble vitamin. It is also called as B2 vitamin. Foods like pork, eggs,…
Q: The limit for G-200 beads is 5000-600,000. When you pass two proteins- Protein A (75, 000), Protein…
A: Proteins are amazing molecules that make everything simple inside the cell. Proteins molecules are…
Q: Which of the following samples are expected to cause a decrease in absorbance over time when…
A: Ensuring adequate light reaching a detector by using water colloidal solutions in light or X-ray…
Q: List the three (3) types of RNA molecules and in one sentence each describe their functions.
A: RNA is Ribo Nucleic acid which is also one of the essential biomolecules essential for life. It is…
Q: 1) If a mutation caused an alanine to turn in glycine, what kind of effect do you think that would…
A: Alanine: An aliphatic amino acid with methyl (-CH3) group side chain and is non-reactive side chain…
Q: 1. Glucose synthesis was investigated in isolated hepatocytes. Different amino acids were added to…
A: Amino acids are building blocks of proteins which is consist of amine group, carboxylic group,…
-
Begining with 1 M concentrations of each reactant and product at pH=7 and 25.0 degrees C, calculate the K'eq of the reaction Pyruvate + NADH <=> Lactate + NADH+H+.
Note the temperature of this reaction will not affect the standard reducton potential delta E'o in the table 13-7b.
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- Begining with 1 M concentrations of each reactant and product at pH=7 and 25.0 degrees C, calculate the K'eq of the reaction Pyruvate + NADH Lactate + NADH+H+. Note the temperature of this reaction will not affect the standard reducton potential delta E° in the table 13-7b.Begining with 1 M concentrations of each reactant and product at pH=7 and 25.0 degrees C, calculate the K'eq (to one decimal point) of the reaction Pyruvate + NADH+H+ <=> Lactate + NAD+.Note the temperature of this reaction will not affect the standard reducton potential delta E'o in the table 13-7b. please provide a comprehensive explanation with each step taken.Begining with 1 M concentrations of each reactant and product at pH=7 and 25.0 degrees C, calculate the K'eq of the reaction Pyruvate + NADH Lactate + NADH+H+. Note the temperature of this reaction will not affect the standard reducton potential
- The standard reduction potential for ubiquione (A or coenzyme Q) is .045 V, and the standard reduciton potential (E) for FAD is -0.219 V. Using these values, show that the oxidation for FADH2 by ubiquinone theoretically liberates enough energy to drive the synthesis of ATP. Faraday constant =96.48KJ/Vol delta G' standard for ATP Synthesis is +30.5 KJ/mol R=8.314 J/mol K=1.987 cal/mol KIf a 0.1 M solution of glucose 1- phosphate at 25 °C is incubated with a catalytic amount of phosphoglucomutase, the glucose 1-phosphate is transformed to glucose 6-phosphate. At equilibrium, the concentrations of the reaction components are Calculate Keq and ΔG′° for this reaction.Standard redox potentials Eo' for some common compounds in biochemistry: Eo' (Volt) + 0,81 +0,42 + 0,25 12 O₂ + 2 H+ + 2 e NO3 + 2 H+ + 2 e 2 cyt c (ox) + 2 e 2 cyt b (ox) + 2 e Pyruvate + 2 H+ + 2 e* NAD + 2 H+ + 2 e Acétoacétate + 2 H+ + 2 e (2) acétoacétate + NADH + H+ H₂O NO₂ + H₂O 2 cyt c (red) 2 cyt b (red) Lactate (3) 2 cyt c (ox) + 2 cyt b (red) NADH + H+ B-hydroxybutyrate + 0,08 - 0,19 With the help of the above table, determine the spontaneous direction of the following reactions in the standard conditions. Justify your answers by calculating the Gibbs (free) energy of reaction in each case. (1) pyruvate + ß-hydroxybutyrate -0,32 - 0,35 lactate + acétoacétate B-hydroxybutyrate + NAD+ 2 cyt c (red) + 2 cyt b (ox)
- Given the following data, calculate Keq for the denaturation reaction of the protein β-lactoglobin at 25oC: ΔH° = –88 kJ/mol ΔS° = 0.3 kJ/mol. The free energy of hydrolysis of ATP in systems free of Mg2+ is −35.7 kJ/mol. When the concentration of this ion is 5 mM, ΔG°observed is approximately −31 kJ/mol at pH 7 and 38°C. Suggest a possible reason for this effect.Calculate AG for this reaction under the following conditions: 37°C, pH 7, [Pyruvate] = [CO₂] = 4.0 mM, [OAA] = 2.0 mM, [ATP] = 3.5 mM, [P;] = 5.0 mM, and [ADP] = 1.8 mM. Use 2 signficant figures. AG= i kJ.mol-1 Under these conditions, the reaction is (Tolerance is +/- 2%)The reactionPyruvate-(aq) + NADH(aq) + H+(aq) → lactate-(aq) + NAD+(aq)where NAD+ is the oxidized form of nicotinamide adenine dinucleotide, occurs in muscle cells deprived of oxygen during strenuous exercise and can lead to cramp. Calculate the biological standard Gibbs energy, ΔrG⊕, for the reaction at 310 K given that the thermodynamic standard reaction Gibbsenergy ΔrGΘ = -66.6 kJ mol-1.
- Calculate the standard free-energy change of the reaction catalyzed by theenzyme phosphoglucomutase, given that, starting with 20 mM glucose 1-phosphate and no glucose 6- phosphate, the final equilibrium mixture at 25 °C and pH 7.0 contains 1.0 mM glucose 1-phosphate and 19 mM glucose 6-phosphate. Does the reaction in the direction of glucose 6-phosphate formation proceed with a loss or a gain of free energy?a) Calculate the enzyme and specific activity of a reaction with 3 pM Hsp90 using the following information: The rate is measured in a spectrophotometer as 0.028 OD units/min in a 1 ml reaction volume. The absorbance was detected at 340nm and the extinction coefficient for NADH at this wavelength is 6200L M- 1 min-1 and the molecular mass of Hsp90 is 82.7 kDa. The rate of NADH utilisation is equivalent to the rate of ATP utilised by Hsp90. Show all your calculations and the units for your answers. b) Calculate the turnover number for the reaction described in (a) aboveConsider the two half-reactions below and their standard reduction potentials. NAD+ + H+ + 2e → NADH Elo= -0.32 V a-Ketoglutarate + CO₂ + 2H+ + 2e → Isocitrate E' = -0.38 V (a) What is AE" for the spontaneous redox reaction that is, the reaction that actually occurs under standard biochemical conditions (pH 7)? (b) Which of the following statements are correct under standard biochemical conditions? i. The concentration of H+ is 1.0 M. ii. The reaction NAD+ + Isocitrate → NADH + H+ + a-Ketoglutarate + CO₂ is favor- able. iii. NAD+ accepts electrons from isocitrate. iv. The NAD → NADH reaction actually occurs in reverse. The a-ketoglutarate → isocitrate reaction occurs as written. (c) Calculate AG" for the reaction in (a). (pH 7, 25°C, pressure, 1 atm.) (d) Suppose that the actual conditions are T = 25°C, pH = 7, CO₂ = 1 atm, [a-Ketoglutarate] 10 mM, [NAD+] = 2.5 mM, and [NADH] = 0.5 mM. PAR = 2 mM, [Isocitrate] DE What is the value of AG under those conditions? (Hints: pH 7 is already…