An organism no longer needs to express a particular gene. What is one strategy it might use of the methods discussed in class? Select all that apply. de-methylate C bases in the genome acetylate C bases in the genome methylate C bases in the genome acetylate histones de-acetylate histones de-acetylate C bases in the genome
Q: "de 96 5 no
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- . Describe FISH analysis is used in findingspecific DNA sequences in a chromosomeGive typing answer with explanation and conclusion The use of which type of endonuclease is more advantageous for genetic cloning: endonucleases generating blunt ends or sticky ends?what is ecDNA? What is its role in cancer? Give appropriate references to support.
- Create your own concept map on vectors used in rDNA technologyDifferentiate between TWO of the following pairs: Genetic and a restriction map Southern and colony blotting Polymerase chain reaction (PCR) and cloningUse the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. F the EcoRI/BamHI double digest produces 3 fragments with only two sizes, what are their sizes? 2.5 kb, 250 b and 250 b 3.0 kb 2.0 kb 500 b and 500 b 3.0 kb 1.0kb and 1.0 kb
- Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. If the Nco1/EcoRI double digests produces 3 fragments what are their sizes? 2.5 kb, 250 b and 250 b 3.0 kb 2.0 kb, 500 b and 500 b 3.0 kb, 1.0kb and 1.0 kbA researcher used polymerase chain reaction (PCR) to test four individual nematodes for the presence of a mutation in Gene Y. When amplified, the fragments of DNA containing the mutated gene alleles were 250 base pairs long and the normal (wild type) alleles were 350 base pairs long. To identify the genotypes of the individual nematodes, the researcher used gel electrophoresis to visualize the PCR results. 1.000 bp 500 bp 300 bp 200 bp 100 bp Remember that these nematodes are diploid organisms and as such, inherited two copies of Gene Y. Which of the following statements is a correct analysis of these results a) Nematode As homozygous for this gene and has two copies of the mutated allele. O b) Nematode C is heterozygous for this gene and has one copy of the mutated allele and one copy of the normal (wild type) allele. O) Nematode D is heterozygous for this gene and has one copy of the mutated allele and one copy of the normal (wild type) allele. ) d) Nematode B is homozygous for this…Translate the sequence of bases in the previous question, starting at the second base.
- Select the definition of a microsatellite. short pieces of DNA surrounding the periphery of the nucleus short pieces of DNA that flank most genes segments of DNA cut by restriction enzymes small DNA sequences that are repeated consecutively a few times to many times O pieces of DNA that can move through the genome by transposition How are microsatellites used in genetic studies? to identify individuals within a population used as markers in the sequencing of the human genome to identify genetic variants in genome-wide associations studies to test if a population is in Hardy–Weinberg equilibrium to transfer genes between organisms O OUsing the diagram below describe the significance of DNA sequencing for identification of mutations.In order to construct a genome library, which statement most accurately describes the correct order of steps in the process? Fragment chromosomal DNA, digest plasmid vector, ligate genomic DNA and digested plasmid vector, transform ligation into bacteria, select for plasmid marker Fragment chromosomal DNA and plasmid vector, ligate genomic DNA and plasmid vector, transform ligation into bacteria, select for gene marker Fragment chromosomal DNA and plasmid vector, ligate genomic DNA and plasmid vector, transform ligation into bacteria, select for plasmid marker Fragment chromosomal DNA, ligate genomic DNA and plasmid vector, transform ligation into bacteria, select for plasmid marker