The replication bubble shown has a number of RNA primers (blue) bound to the two strands of the unwound double helix. In some areas of the replication bubble, new DNA (red) has begun to be synthesized. 3' A Replication fork movement D Origin of replication (on) B C Replication fork movement Which of the following statements is correct? 1.DNA polymerase III will continue extending the DNA at the location indicated by arrow B. 2.At some point during the replication process, the enzyme ligase will be needed at the location indicated by arrow D. 3.At some point during the replication process, DNA polymerase I will add bases at the location indicated by arrow C. 4.Arrows A and D are both pointing to a 3' end of RNA.

Assume a deletion occurs in a gene that encodes DNA polymerase I and no functional DNA polymerase I is produced.  What will be the most likely consequence of this mutation?     The DNA would not exist in a supercoiled state.     There would be no RNA primers laid down.     The DNA will not be able to unwind to initiate replication.     The DNA strands would contain pieces of RNA.     There would be no DNA replication on the leading or lagging strands.

Replication is occurring normally in these cells; would you expect to find a primer in both positions? Why or why not? The beginning of the hexose kinase gene's sequence can be found below, the +1 nucleotide is underlined and bolded. It also contains an origin of replication (ORI) which is found at position 30. 20 ORI 40 60 5'...TTCGAGCTCTCGTCGTCGAGATACGCGATGATATTACTGGTAATATGGGGATGCACTATC...3’ 3' ...AAGCTCGAGAGCAGCAGCTCTATGCGCTACTATAATGACCATTATACCCCTACGTGATAG...5’ promoter

Can you please answer number 15

All known DNA polymerases catalyze synthesis only in the 5' → 3' direction.  Nevertheless, during semiconservative DNA replication in the cell, they are able to catalyze the synthesis of both daughter chains, which would appear to require synthesis in the 3' → 5' direction on one strand.  Explain the process that occurs in the cell that allows for synthesis of both daughter chains by DNA polymerase

(a) What is the function of helicase in DNA replication?(b) What is the function of DNA polymerase?(c) What are replication forks? Compare and contrast leading and lagging strands. Answer all please

a. What process would be unaffected in with defective topoisomerase? Prokaryotic DNA packaging Eukaryotic DNA packaging Prokaryotic DNA replication Eukaryotic DNA replication All of the above rely on topoisomerase function b. Why is primase required for DNA replication? To unwind the DNA helix To prime nucleotides for addition to the growing chain To provide a 3' OH for DNA polymerase To recognize the origin of replication To remove supercoils ahead of the replication machinery C. What is the purpose of PCR reaction? To alter the sequence of a fragment of DNA. To insert a fragment of DNA into a bacterium To amplify a fragment of ONA To destroy a fragment of DNA To determine the sequence of nucleotides in a fragment of DNA.

Replication of a circular DNA molecule can occur by either theta () replication or by rolling circle replication. Describe or explain three differences between these two modes of DNA replication (you must accurately describe differences between the two approaches to replication - an accurate statement about one form of replication that does not clearly indicate how the other form differs is not a correct)

What is true of this figure? (can be multiple answers) a. the replication fork is asymmetrical b. the DNA strands are anti-parallel c. one strand runs 3’ to 5’ d. one strand runs 5’ to 3’ e. both strands have identical bases

Would my anwser be correct for this question?