In(C) 5.00 4.00 3.00 2.00 1.00 0.00 -1.00 -2.00 In(R) In(C) Oral (po) Terminal Line Regression 5.00 4.00 3.00 y=-0.468x+3.395 R2 0.9999 D 2.00 1.00 0.00 -1.00 -2.00 0 2 3 4 5 6 t (hr) Oral (po) Residual Line Regression y -2.1135x+3.4547 R²=0.9912 3 S t (hr) Intraperitoneal (ip) Terminal Line Regression y -0.5171x+4.038 R2=0.9999- 5.00 4.00 3.00 D ° 2.00 1.00 0.00 -1.00 -2.00 0 2 3 t (hr) 5.00 4.00 3.00 2.00 1.00 0.00 -1.00 In(R) -2.00 Intraperitoneal (ip) Residual Line Regression y=-1.7873x+4.0604 R2 0.9983 0 2 3 t (hr) 1. In vivo testing provides valuable insight into a drug's kinetics. Assessing drug kinetics following multiple different routes of administration provides greater insight than just a single route of administration alone. The following data was collected in 250 g rats following bolus iv, oral (po), and intraperitoneal (ip) administration. 10 mg/kg bolus iv 500 mg/kg po or ip Time (hr) Civ (mg/L) Time (hr) Cpo (mg/L) Cip (mg/L) 0.083 2.679 0.00 0.00 0.00 0.50 1.988 0.25 7.87 12.73 1.0 1.359 0.50 11.19 18.98 2.0 0.730 1.0 15.07 24.32 3.0 0.401 2.0 11.64 20.02 3.0 7.32 12.02 4.0 4.63 7.27 6.0 1.79 2.53 From this data, the following graphs were created and linear regression lines determined. You should practice making these graphs, but are not required to make them. In(C) Bolus IV Terminal Line Regression 5.00 4.00 3.00 2.00 1.00 y -0.6489x+1.0045 R²=0.9979 0.00 -1.00 -2.00 0 1 2 3 5 6 t (hr)

1. In vivo testing provides valuable insight into a drug’s kinetics. Assessing drug kinetics following multiple different routes of administration provides greater insight than just a single route of administration alone. The following data was collected in 250 g rats following bolus iv, oral (po), and intraperitoneal (ip) administration.Using this data and set of graphs, determine: (a) k, C0, V, and AUC* for the bolus iv data  (b) k, ka, B1, and AUC* for the po data  (c) k, ka, B1, and AUC* for the ip data  (d) relative bioavailability for po vs ip, Fpo/Fip  (e) absolute po bioavailability, (f)Fpo absolute ip bioavailability, Fip    MAKE SURE ANSWERS HAVE UNITS if appropriate. SHOW ALL WORK, including equation used, variables used and each step to your solution.