FALL 2020 WEEK 6 - Macromolecules SSA

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Utah Valley University *

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1010BB

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Chemistry

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Apr 3, 2024

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docx

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WEEK 6 – MACROMOLECULES SSA- Standard Curve (10 Points) Create a standard curve in Excel using the following data. Copy and paste your graph into the space below. Before you copy it from Excel, be sure your graph includes: A trend line the linear equation R 2 value labeled x & y axes (including title and units) Figure caption [INSERT STANDARD CURVE GRAPH BELOW] 0 0.5 1 1.5 2 2.5 -0.5 0 0.5 1 1.5 2 f(x) = 0.77 x − 0.17 R² = 0.79 Protein concentration (mg/mL) Absorbance( 595 nm) Figure 1. This shows the concentration and absorbance with a standard graph line. Use your standard curve to complete the following statements: The absorbance of Unknown A is 0.455 nm. The protein concentration of Unknown A is calculated to be .51680 mg/ml using the standard curve. The absorbance of Unknown B is 0.826nm. The protein concentration of Unknown B is calculated to be .801612 mg/ml using the same standard curve. Unknown B contains a higher concentration of protein than Unknown A Absorbance Data for Protein Standards Protein Concentration (mg/mL) Absorbance 595 nm 2.00 1.489 1.50 1.022 1.00 0.697 0.750 0.623 0.500 0.458 0.250 0.233 0.125 0.121 0 0
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