PhysioEx Exercise 1 Activity 1 - Rickey Maung

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1/28/2022 PhysioEx Exercise 1 Activity 1 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act1/ 1/4 PhysioEx Lab Report Exercise 1: Cell Transport Mechanisms and Permeability Activity 1: Simulating Dialysis (Simple Diffusion) Name: Cell Transport Activity 1 Results - Rickey Maung Date: 28 January 2022 Session ID: session-e12eb868-a74f-91c7-3d1c-527b18cb3142 Pre-lab Quiz Results You scored 100% by answering 3 out of 3 questions correctly. Experiment Results Predict Questions Stop & Think Questions The driving force for diffusion is You correctly answered: the kinetic energy of the molecules in motion. 1 In diffusion, molecules move You correctly answered: from high concentration to low concentration. 2 Which of the following dialysis membranes has the largest pore size? You correctly answered: 200 MWCO. 3 Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane? Your answer: No, not at all. 1 Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average molecular weight of a single amino acid is 135 g/mol. Which of the following will be able to diffuse through the 200 MWCO membrane? Your answer: glucose only. 2 The reason sodium chloride didn't diffuse left to right is that You correctly answered: the membrane pore size was too small. 1
1/28/2022 PhysioEx Exercise 1 Activity 1 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act1/ 2/4 Experiment Data Solute MWCO Left Solute (mM) Right Solute (mM) Average Diffusion (mM/sec) Na Cl 20 9.00 0.00 0.0000 Urea 20 0.00 0.00 0.0000 Albumin 20 0.00 0.00 0.0000 Glucose 20 0.00 0.00 0.0000 Na Cl 20 0.00 0.00 0.0000 Urea 20 9.00 0.00 0.0000 Albumin 20 0.00 0.00 0.0000 Glucose 20 0.00 0.00 0.0000 Na Cl 50 9.00 0.00 0.0150 Urea 50 0.00 0.00 0.0000 Albumin 50 0.00 0.00 0.0000 Glucose 50 0.00 0.00 0.0000 Na Cl 50 18.00 0.00 0.0273 Urea 50 0.00 0.00 0.0000 Albumin 50 0.00 0.00 0.0000 Glucose 50 0.00 0.00 0.0000 Na Cl 100 9.00 0.00 0.0150 Urea 100 0.00 0.00 0.0000 Albumin 100 0.00 0.00 0.0000 Glucose 100 0.00 0.00 0.0000 Na Cl 100 0.00 0.00 0.0000 Urea 100 9.00 0.00 0.0094 Albumin 100 0.00 0.00 0.0000 Glucose 100 0.00 0.00 0.0000 Na Cl 200 0.00 0.00 0.0000 Urea 200 0 00 0 00 0 0000 Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino acid is 135 g/mol. There is no reason to run these solutes at the 20 MWCO because You correctly answered: glucose and albumin are both too large to pass. 2 The rate of diffusion for urea You correctly answered: is slower than that for sodium because urea is a larger molecule. 3 + - + - + - + - + - + - + -
1/28/2022 PhysioEx Exercise 1 Activity 1 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act1/ 3/4 Urea 200 0.00 0.00 0.0000 Albumin 200 0.00 0.00 0.0000 Glucose 200 9.00 0.00 0.0042 Na Cl 200 0.00 0.00 0.0000 Urea 200 0.00 0.00 0.0000 Albumin 200 9.00 0.00 0.0000 Glucose 200 0.00 0.00 0.0000 Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. Review Sheet Results + - The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left beaker was to You correctly answered: increase the rate of diffusion. 1 Describe the difference between the rate of diffusion seen for sodium and urea. You correctly answered: Urea diffused more slowly because it is larger than sodium. 2 Which of the following solutes did not pass through any of the membranes? You correctly answered: albumin. 3 When diffusion stops, we say the solution has reached You correctly answered: equilibrium. 4 Describe two variables that affect the rate of diffusion. Your answer: The solute size and pore size both affects the rate of diffusion. 1 Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? Your answer: Urea was not able to diffuse through the 20 MWCO membrane because its molecular weight is larger than what the 20 MWCO membrane can support. Compare the Urea against the 100 MWCO membrane and it was able to diffuse through it because its molecular weight of 60.07 at that MWCO is able to be supported. 2
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1/28/2022 PhysioEx Exercise 1 Activity 1 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act1/ 4/4 Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? Your answer: Only glucose was able to diffuse through the 200 MWCO membrane, albeit very slowly due to its large size. Comparatively, albumin is even larger and more complex than glucose and was still unable to pass through the 200 MWCO membrane. 3 Put the following in order from smallest to largest molecular weight: glucose, sodium chloride, albumin, and urea. Your answer: Sodium chloride, Urea, Glucose, Albumin. 4