PhysioEx Exercise 1 Activity 1 - Rickey Maung
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1/28/2022
PhysioEx Exercise 1 Activity 1
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PhysioEx Lab Report
Exercise 1: Cell Transport Mechanisms and Permeability Activity 1: Simulating Dialysis (Simple Diffusion) Name: Cell Transport Activity 1 Results - Rickey Maung Date: 28 January 2022 Session ID: session-e12eb868-a74f-91c7-3d1c-527b18cb3142 Pre-lab Quiz Results
You scored 100% by answering 3 out of 3 questions correctly.
Experiment Results
Predict Questions
Stop & Think Questions
The driving force for diffusion is
You correctly answered: the kinetic energy of the molecules in motion.
1
In diffusion, molecules move
You correctly answered: from high concentration to low concentration.
2
Which of the following dialysis membranes has the largest pore size?
You correctly answered: 200 MWCO.
3
Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse
through the 20 MWCO membrane?
Your answer: No, not at all.
1
Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607
amino acids, and the average molecular weight of a single amino acid is 135 g/mol. Which of the following will be able to diffuse through the 200 MWCO membrane?
Your answer: glucose only.
2
The reason sodium chloride didn't diffuse left to right is that
You correctly answered: the membrane pore size was too small.
1
1/28/2022
PhysioEx Exercise 1 Activity 1
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Experiment Data
Solute
MWCO
Left Solute (mM)
Right Solute
(mM)
Average
Diffusion
(mM/sec)
Na
Cl
20
9.00
0.00
0.0000
Urea
20
0.00
0.00
0.0000
Albumin
20
0.00
0.00
0.0000
Glucose
20
0.00
0.00
0.0000
Na
Cl
20
0.00
0.00
0.0000
Urea
20
9.00
0.00
0.0000
Albumin
20
0.00
0.00
0.0000
Glucose
20
0.00
0.00
0.0000
Na
Cl
50
9.00
0.00
0.0150
Urea
50
0.00
0.00
0.0000
Albumin
50
0.00
0.00
0.0000
Glucose
50
0.00
0.00
0.0000
Na
Cl
50
18.00
0.00
0.0273
Urea
50
0.00
0.00
0.0000
Albumin
50
0.00
0.00
0.0000
Glucose
50
0.00
0.00
0.0000
Na
Cl
100
9.00
0.00
0.0150
Urea
100
0.00
0.00
0.0000
Albumin
100
0.00
0.00
0.0000
Glucose
100
0.00
0.00
0.0000
Na
Cl
100
0.00
0.00
0.0000
Urea
100
9.00
0.00
0.0094
Albumin
100
0.00
0.00
0.0000
Glucose
100
0.00
0.00
0.0000
Na
Cl
200
0.00
0.00
0.0000
Urea
200
0 00
0 00
0 0000
Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average
molecular weight of a single amino acid is 135 g/mol. There is no reason to run these solutes
at the 20 MWCO because
You correctly answered: glucose and albumin are both too large to pass.
2
The rate of diffusion for urea
You correctly answered: is slower than that for sodium because urea is a larger molecule.
3
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-
+
-
+
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+
-
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1/28/2022
PhysioEx Exercise 1 Activity 1
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Urea
200
0.00
0.00
0.0000
Albumin
200
0.00
0.00
0.0000
Glucose
200
9.00
0.00
0.0042
Na
Cl
200
0.00
0.00
0.0000
Urea
200
0.00
0.00
0.0000
Albumin
200
9.00
0.00
0.0000
Glucose
200
0.00
0.00
0.0000
Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
Review Sheet Results
+
-
The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left
beaker was to
You correctly answered: increase the rate of diffusion.
1
Describe the difference between the rate of diffusion seen for sodium and urea.
You correctly answered: Urea diffused more slowly because it is larger than sodium.
2
Which of the following solutes did not pass through any of the membranes?
You correctly answered: albumin.
3
When diffusion stops, we say the solution has reached
You correctly answered: equilibrium.
4
Describe two variables that affect the rate of diffusion.
Your answer:
The solute size and pore size both affects the rate of diffusion.
1
Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How
well did the results compare with your prediction?
Your answer:
Urea was not able to diffuse through the 20 MWCO membrane because its molecular weight is larger than what the 20 MWCO membrane can support. Compare the Urea against the 100 MWCO membrane and it was able to diffuse through it because its molecular weight of 60.07 at that MWCO is able to be supported.
2
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PhysioEx Exercise 1 Activity 1
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Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO
membrane. How well did the results compare with your prediction?
Your answer:
Only glucose was able to diffuse through the 200 MWCO membrane, albeit very slowly due to its large size. Comparatively, albumin is even larger and more complex than glucose and was still unable to pass through the 200 MWCO membrane.
3
Put the following in order from smallest to largest molecular weight: glucose, sodium
chloride, albumin, and urea.
Your answer:
Sodium chloride, Urea, Glucose, Albumin.
4
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