Pipette

Calibrating A Volumetric Pipette Ryane Washington Quantitative Analysis Lab INTRODUCTION This experiment was conducted to learn the relationship of 3 different types of pipettes and how to be accurate at measuring volumes of substances very accurately in quantitative analysis lab work. The accuracy of the measurement is the degree of closeness of measurements of a quantity’s actual volumes and the precision is the degree to which that are repeated measurements that are not changed show the

to which commercially bought pipettes accurately deliver desired volumes. A range of small and large volumes of DI water were drawn using three different sized pipettes and massed using an analytical balance. The weights of the water aliquots were divided by the appropriate density of water at 26 to calculate the actual volumes delivered. Error percent analysis was then utilized to deduce the deviation from the accurate volumes. The three different sizes of pipettes used were Epprendorf 10, 100 and

precision of class A pipettes. The tolerance for class A pipettes is ±.006 for 1mL, ±.01 for 5 mL, and ± .02 for 10 mL (. As a whole the class did rather well in terms of the expected accuracy and precision of the class A glassware. I found the expected values from a website. The student who had the best calibration was student number 1. There recorded values were within the closest range of each pipette. I believe that my data is not within the best simply because, one; my one mL pipette was not delivering

Title: Laboratory Techniques & Measurements Purpose: To become familiar with the International System of Units and common laboratory equipment and techniques. To learn how to determine volume, mass, length, and temperature of a wide variety of items. To learn how to calculate density and concentration of dilutions. Procedure: I used a ruler, thermometer, and scale to take measurements. I used a graduated cylinder, short step pipet, scale, and ruler to determine volume and density. I used a

i. The Objectives of Part A of the experiment include (1) to determine the accuracy and precision of the variation in mass across packets of sweetener (2) to determine the accuracy and precision variation in mass across balances (3) to gain a relative understanding of the accuracy and precision of such values by both comparing the values to each other, as well as to the Nominal mass of the sweetener (4) to therefore determine the difference by finding the value of the mean and the standard deviation

4. Fill the pipette 5ml of 0.02 molL-1 NaCl using the pipette filler and repeat step 3, so that 0.02 molL-1 NaCl touches all the sides. Discard the NaCl. 5. Repeat step 4, two more times 6. Now rinse the funnel with deionised water. 7. Using the funnel pour the deionised water into the burette up to the halfway mark, don’t forget to turn off the tap. 8. Rinse the burette in the same manner as the pipette; hold it horizontally and rotate, so that the water makes contact with all the side. Then let

precautions. The students then put one pipette of glucose and one pipette of yeast into a test tube. The instructor filled a 250 ml beaker at each station filled with 37° Celsius water. Next, the test tube with the glucose and yeast solution was placed in the beaker to incubate for 10 minutes. After the 10 minutes had passed, a clean pipette was used to remove 1 ml of the solution from the test tube and place it in a 500 ml plastic respiration chamber. The pipette was the thrown away. Next, the CO2 gas

types of pipettes to measure the volumes of water. The accuracy of pipetting could be determined by the weight of liquid we dispensed and used it to compare with expected weight. The purpose of this lab was to use pipets correctly, also increased the precision and accuracy for the lab demand. Methods and Materials: We used both 5mL and 10 mL pipettes, a pipette bulb, a 50mL dry beaker, water and scales. We did the experiment for three parts, we used 5mL pipette twice and used 10mL pipette once. The

To calculate the expected number of pipette tips needed per month take the number of pipette tip per sample to multiply it with the average sample analysed per month, 5 x 100 = 500. Therefore, the expected number of pipette tips needed per month would be 500 plastic pipette tips. b) Laboratory ABC uses both glass pipettes (1mL to 5mL) and disposable plastic pipette tips- yellow (20μL), blue (1000μL) and white (5mL). Glass pipettes and white pipette tips are currently washed and reused. Blue and

accomplished by measuring O2 production with different color tubes. For the cellular respiration experiment, the stock yeast culture was stirred before used. Pipette 10 mL yeast suspension into each of 7 large diameter test tubes. Cap the tube with the rubber stopper and pipette. Make a documentation of the starting point of the solution in the pipette. Next, add a volume of each different sugar solution, glucose, fructose, lactose, galactose, sucrose, and honey to one of the six test tubes, sufficient