Expressed sequence tag

where it can produce oil and table olives. Gene at certain tissue in species will be expressed where cDNA are sequenced to produce expressed sequence tag (EST) library. EST database is beneficial as it allows new gene discovery, marker discovery, gene mapping, and functional studies to be carried out. This research has contributed EST library of 2304 clone sequences from the young olive leaf and 1536 clone sequences from the immature olive fruit for Turkish olive cultivar Gemlik. Good quality ESTs

Plant Biology Journal entitled “Gene discovery in the wood-forming tissues of poplar: Analysis of 5,692 expressed sequence tags”, by Sterky et al., 1998, expressed sequence tags (EST) database was prepared with specific procedure. First of all, EST can be defined as a short fragments sequence, range from 100 bp- 1000 bp. It derived from a c DNA clone which represents the expressed genes sequence. Usually, ESTs are used to identify the full length genes and serves as a target for mapping with providing

Expressed Sequence Tags (ESTs) versus Serial Analysis of Gene Expression (SAGE) INTRODUCTION Expressed sequence tag or also known as EST are 200 to 800 unedited nucleotide bases in length, and randomly selected single pass sequence reads which are derived from the cDNA libraries while serial analysis of gene expression or SAGE have more shorter sequence tags with only 10 to 20 base pairs. But even it is short it still have enough information to uniquely identify a transcript, especially if it is

All cells in the body contain the same DNA sequence. Although all cells contain an identical DNA sequence, they all have different cell structures and functions. Each structure and function of the cell differs because the cell has specific use for or will only express certain genes. Genes in DNA are expressed when they are read and transcribed into RNA which then gets translated into proteins by ribosome structures. Proteins are used to determine a cells characteristics and functions. Inside of each

et al., 1991). His tags are commonly used to purify proteins through immobilized metal-affinity chromatography (IMAC) (Lilius et al., 1991). This rapid and efficient method separates the recombinant protein from unwanted products such as RNA. The His tag DNA sequence is inserted into the

Sequence specific primer PCR With the advancement of sequencing technology, vast information on DNA sequences of many animal genomes has been developed (Goff et al. 2002; Yu et al. 2002). In order to correlate DNA sequence information with particular a phenotype of a trait, sequence-specific molecular marker techniques have been designed. Expressed sequence tags (EST) analysis is one such type. By adopting this method, Expressed sequence tags of many animal species have been created and these sequences

What is Epigenetics? The word “epigenetic” means “in addition to changes in genetic sequence.” The term has changed to include any process that alters gene activity without changing the DNA sequence, and leads to modifications that can be transmitted to daughter cells. The term epigenetics was first used in the early 1940s, when embryologist Conrad Waddington used it to describe the interactions between genes and their products, which give rise to an organism’s observable characteristics or phenotype

Indirect enzyme-linked immunosorbent assay, a competitive ELISA will be conducted to detect Babesia antibodies in serum of naturally infected cattle based on Babesia lysate, Cyanogen bromide-activated Sepharose 4B, affinity purified antigen and expressed,

phosphate and sugar groups linked to a chain of nucleotides. DNA is important because it gives instructions to an organism on how to develop, survive, and reproduce through DNA sequences. These sequences contain instructions on how to make proteins, which are complex molecules that do most of the work in the human body. DNA Sequences that contain instructions on how to make proteins are genes which also determine physical traits. The complete DNA instruction book, or genome, for a human, contains about

org/biointeractive/gene-switch 1. What does the yellow part of the DNA represent? The yellow part of the DNA is use for coding. It only expressed if RNApolymerase lands on gene’s promotor and makes mRNA from the gene. 2. What does the pink part of the DNA represent? The DNA promoter 3. What needs to land on the pink part in order for the gene to be expressed? RNA Polymerase 4. What do the blue, red and green parts of the DNA represent? Regulator switches 5. What are the functions of the blue