This lab experiment serves as a model for community succession using bacterial colonies as the model. A bacterial colony grows from a single bacterium and is composed of millions of cells. Each colony has distinctive colony morphology: size, shape, color, consistency, and color. Community succession is a phenomenon observed in the organizational hierarchy of all living organisms. Community succession is not limited to bacterial colonies, but spans the entire community of life. As the community
The Advent of Penicillin The advent of penicillin forever changed the world of medicine at its discovery with its ability to treat diseases, deadly at the time, that are now considered commonplace and easily treatable. Penicillin was one of the greatest discoveries of the twentieth century, as antibiotics are one of the most highly prescribed drugs in the world today. Although its discovery is often described as serendipitous, the process by which it was cultivated was quite meticulous, and
On the first day of testing, a gram stain test, a KOH test, a catalase test, and an oxidase test were completed. The gram stain test indicated that the unknown species is a gram positive rod, and the bacteria showed no strings during the KOH test confirming that the species is gram positive. From these two tests, the unknown was concluded to be in the family Bacillicae. The bacteria remained yellow on the cotton swab even after the oxidase reagent was dropped onto the swab showing that the bacteria
is an outer lipid layer that is closed off by the LPS, or lipopolysaccharide. This is a thicker capsule than in gram positive bacterial cells. The counterstain, which is known as safranin, is used to stain the gram-negitive cells. This technique shows the difference between gram positive and gram negitive cells that will allow for experimentation of these different bacterial cell
Bio Pre Proposal I. OBJECTIVE: To have the ability to identify gram positive and gram negative cells and understand how the staining affects them. II. HYPOTHESIS: if a smear of both Escherichia coli and Staphylococcus epidermidis are stained, then gram negative and gram positive stains should result respectively and be identifiable under the microscope due to the structure of the cell walls. III. PROCEDURE: 1. Obtain a blank, sterile microscope slide. Using an inoculating loop add one drop of distilled
Purpose: To understand the difference between Gram positive and Gram negative bacteria. We also used this lab to examine our mixed culture, which had been Gram stained, under the microscope. Theory and background The bacterial cell wall is the outer layer of the cell that aids in structural support and protection from the outside environment. Bacteria can be identified by the structure of their cell wall and classified into two groups known to have different cell wall types. Two of these types
Bacteria can be divided into two types of species, gram positive bacteria and gram negative bacteria. To determine whether a certain type of bacteria was classified tests were conducted by Hans Christian Gram; who discovered the differences within the cell walls of the bacteria. Gram positive bacteria have a thick wall which consists of the protein peptidoglycan. This is opposite to gram negative bacteria which has a thin cell wall, which fold over each other (Wells, 2017). They retain a much thinner
On a still, warm morning our class set out on a hike to collect water and soil samples to explore. Our water sample was collected from the first portion of Sycamore Creek located on the Los Penasquitos Trail in Sorrento Valley. Collecting 6 in. deep into the creek, we wanted to ensure that the sample in the plastic test tube included soil, algae and vegetation from the floor. The sample was allowed to sit uncapped and covered with a layer of film for 24+ hours before microscopic observation occurred
Problem: An unknown bacteria in test tube #13 needs to be identified out of the list of bacteria provided as possible. Hypothesis: If different tests were carried out to identify the bacteria’s characteristics, then the bacteria could be identified, because a dichotomous key can be used to eliminate all other bacteria in the list Procedure: 1. Obtain an inoculating loop, Bunsen burner, test tube #13 and a test tube rack. 2. Gram Stain Test to determine the shape of the bacteria, it’s orientation
As the flowchart shows, a series of tests were conducted to identify the unknown bacterium #65. Microscopic observation of the gram stain indicated a gram-positive coccus bacterium. S. epidermidis was used as the gram-positive control while E. coli was used as the gram-negative control. This observation led to the elimination of all gram negative and rod-shaped genera: Enterobacter, Citrobacter, Klebsiella, Escherichia, Pseudomonas, Serratia, Alcaligenes, Neisseria, Proteus, Salmonella, Shigella