Body Structures & Functions Updated
Body Structures & Functions Updated
13th Edition
ISBN: 9780357191606
Author: Scott
Publisher: Cengage
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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
When setting up a PCR reaction to act as a negative control for the surface protein A gene... Which primers will you add to the reaction mix? mecA primers, spa primers, mecA primers and spa primers, no primers What will you add in place of template? sterile water, MRSA DNA, Patient DNA, S. aureus DNA
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12 Organ Systems | Roles & functions | Easy science lesson; Author: Learn Easy Science;https://www.youtube.com/watch?v=cQIU0yJ8RBg;License: Standard youtube license