Chapter 9, Problem 13MIC

In paragraph form (about 2-3 long paragraphs), how would you characterize and identify antimicrobial agents from root samples using x-ray crystallography and infrared spectroscopy? Please include the principles and the details and type specifically for the antimicrobial agents of the methodology.

How do eosin-methylene blue (EMB) agar plates work? What organism(s) are they designed to detect? How would a positive test appear on the plate?

How are clinical chemistry test results used to confirm or rule out a presumptive diagnosis? Why is this important and give references.

make the test principle, materials required for the analysis, sample preparation, test procedure, indication of a positive result, complications/interferences, for nitrous acid test.

Solid and liquid media are commonly prepared based on manufacturer’s instructions. If no instruction is provided on how the media should be sterilized, what would be the best option? METHOD:RATIONALE:

Provide the Experimental procedure of the following: Example: Ninhydrin Test -The samples needed in this test were 1% casein solution, albumin and gelatin and the reagent used was ninhydrin (Triketohydrindenehydrate or Triketohydrin hydrate). Test tube, test tube stand, pippete, water bath, and spectrophotometer were the materials and equipment used. Ninhydrin reagent in 1-2 drops was added to 0.5 ml of the sample with care. It was mixed and heated to boiling for 1-2 minutes. Then, it was allowed to cool to record the observation.   Test that needs Experimental procedure: 1. Heat denaturation Samples: Albumin, gelatin   2. Isoelectric Precipitation Samples: Casein   3. Concentrated Mineral Acids Samples: Albumin   4. Organic Solvents Samples: Albumin

Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test.  Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.

Identification of an organism does not require a pure culture because Bergey’s Manual will sort out the contaminant. Identification of an organism does not require a pure culture because Bergey’s Manual will sort out the contaminant. True False

Is it safe to use 100g of Lye (NaOH) as a disinfectant at homes? Justify your claim by computing the percent composition. Claim: Yes/No Solution: