Concept explainers
To answer:
Prevention of protein synthesis by mutated gene through recombinant DNA technology.
Introduction:
In translation, mRNA is converted into amino acid sequences, resulting in protein synthesis. Three important components are involved in translation are m-RNA, t-RNA, and the ribosome. Protein synthesis occurs in the cytoplasm of the cell. The mRNA sequence encoded by the genetic material is translated into a specific protein. The t-RNA binds to free amino acids and transfer them to the ribosome and the amino acids are added to the growing chain of the protein sequence. The ribosome reads mRNA and synthesizes protein based on codons present in the mRNA sequence. The ribosome binds to the anticodon of particular t-RNA according to m-RNA sequence and assembles amino acids corresponding to m-RNA codons. Three
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- Explain why exome sequencing can be almost as valuable as genome sequencing. (Explain in your own words)arrow_forwardA patient is diagnosed with lung cancer using imaging. To establish the best treatment option, doctors ask to profile the gene expression of the tumor, using a biopsy and microarray analysis. Describe the steps involved in the analysis in detail. The analysis reveals that a new gene therapy could be beneficial to the patient. Explain briefly how gene therapy could be used to cure cancer and detail how the treatment can be formulated for delivery to the patient. To prepare DNA for the gene therapy formulation, it is often important to measure the size of the molecule. Explain how this measurement can be done using agarose gel electrophoresis, taking care to detail the property of the DNA molecule at the basis of the technique. To generate large amounts of DNA to manufacture gene therapy payloads or to be able to see them on gel electrophoresis, specific sequences can be amplified by PCR. For a sequence of a 100 base pairs, calculate the number cycles of PCR required to generate 1 ng of…arrow_forwardPractical applications of recombinant DNA technology include the: 1. Efficient production of useful proteins and development of a new type of vaccines 2. Creation of novel genotypes for the synthesis of economically important molecules. 3. Generation of DNA and RNA sequences for use in medical diagnosis. 4. all of the above WHICH OF THE FOLLOWING TECHNIQUE IS NOT USED TO MANIPULATE THE GENOME 1. siRNA 2. ZF (zinc finger) 3. TALE (transcription activator light effector) 4. CRISPR COMMON DISEASE- COMMON VARIANT HYPOTHESIS STATES THAT 1. common disorders are likely influences by genetic variation that is also common in the population 2. if an SNP produced a deleterious mutation and changes amino acid sequence in 40% of individuals- it will produce disease phenotype in 40% individual 3. A SNP produce little change in gene expression- it will produce slight increase in risk/ manifestation of disease phenotype 4. all of the above WHAT IS TRUE REGARDING GENE ORGANIZATION IN THE NUCLEUS…arrow_forward
- Explain why exome sequencing can be almost as valuable as genome sequencing.arrow_forwardBriefly present experimental and practical benefits of using PCR in DNA cloning process. Give some examples of clinical applications.arrow_forwardDescribe the important features of cloning vectors. Explain the purpose of selectable markers in cloning experiments.arrow_forward
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