Choice between fungicide treated pea-seed stock versus the untreated stock, for a crop. Introduction: A fungus is a eukaryotic organism with thousands of species. Many of these species have the ability to act as a pathogen for plants and as a result, can feed on plants. A fungicide is a drug or medicine that can prevent these fungi growing in the plant species.

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Chapter 31, Problem 4S

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To determine: Choice between fungicide treated pea-seed stock versus the untreated stock, for a crop.

Introduction: A fungus is a eukaryotic organism with thousands of species. Many of these species have the ability to act as a pathogen for plants and as a result, can feed on plants. A fungicide is a drug or medicine that can prevent these fungi growing in the plant species.

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In 1971, Dr. Akira Endo, working at the Sankyo company in Tokyo speculated that fungi not only contained antibiotics but also inhibitors of other processes such as cholesterol metabolism. Over a period of several years, he screened more than 6,000 fungal cultures until a positive result emerged. This compound, which was named compactin, came from the blue-green mold Penicillium citrinum Pen-5 that was isolated from a rice sample collected at a grain shop in Kyoto. Based on biochemical assays, compactin is the first known statin. 1. The original kinetics data for compactin inhibition of HMG-CoA reductase enzyme that was published by Dr. Endo in 1976 is shown on the right. a) Determine the Km and Vmax values for enzymatic activity. Do NOT forget the units. Answers can be expressed either as a fraction or in decimals. With No inhibitor: ; Vmax= -60 1/V, (nanomoles/min)-1 0.4 0.35+ 0.3 0.25 0.2 0.15 0.1 0.05 -40 -20 0 + 24 nM compactin + 70 nM compactin None T 20 40 60 1/[HMG-CoA], (mM)-1…