Genetics: Analysis and Principles
5th Edition
ISBN: 9780073525341
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 19.2, Problem 1COMQ
With regard to phage
provide a site where the phage can align with the E. coli chromosome and become inserted there.
attach the phage to the surface of the bacterium.
promote the movement of the phage from one bacterium to another.
facilitate the attachment of phage DNA to phage coat proteins.
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Students have asked these similar questions
phage can be "induced" as a result of DNA damage. Consequences of
induction include.
Temperate phage, e.g lambda phage; lytic cycle and transduction
Lytic T4 phage, assembly, conjugation
O Lytic phage, e.g. T4 phage; lysogenic cycle, prophage formation
Temperate phage, e.g. lambada phage, prophage formation, transformation
O T4 phage, lytic cycle, specialized trasnduction
Classify each statement as true of generalized transduction or specialized transduction.
Generalized transduction
Specialized transduction
Answer Bank
Genes transfer from random sites in the bacterial chromosome.
This utilizes lysogenic phages.
Genes that transfer depend on the prophage insertion site.
This utilizes lytic phages.
Phage inserts itself at a specific site in the bacterial chromosome.
This requires accidental packaging of bacterial DNA in viral capsids.
During the lysogenic cycle of lambda (1) phage
the phage genome is integrated in the host chromosome where it is passed on to daughter cells through binary fission.
the phage DNA is copied and accumulates in the cytoplasm. Then a trigger causes capsid proteins to be made and phages are assembled.
the product of one prophage gene is made and it activates most of the other prophage genes.
the viral genes immediately turn the host cell into a lambda-producing factory. Then the host lyses.
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Chapter 19 Solutions
Genetics: Analysis and Principles
Ch. 19.1 - 1. Homologous recombination refers to the exchange...Ch. 19.1 - During the molecular process of homologous...Ch. 19.1 - 3. A key difference between the original Holliday...Ch. 19.1 - Which of the following mechanisms can cause gene...Ch. 19.2 - With regard to phage , the role of attachment...Ch. 19.2 - 1. During site-specific recombination that occurs...Ch. 19.3 - Which of the following types of transposable...Ch. 19.3 - Prob. 2COMQCh. 19.3 - Prob. 3COMQCh. 19 - 1. Describe the similarities and differences...
Ch. 19 - Prob. 2CONQCh. 19 - 3. Which steps in the double-strand break model...Ch. 19 - Prob. 4CONQCh. 19 - Prob. 5CONQCh. 19 - Prob. 6CONQCh. 19 - Prob. 7CONQCh. 19 - 8. What is gene conversion?
Ch. 19 - Make a list of the differences between the...Ch. 19 - Prob. 10CONQCh. 19 - Prob. 11CONQCh. 19 - 12. According to the double-strand break model,...Ch. 19 - What type of DNA structure is recognized by RecG...Ch. 19 - Briefly describe three ways that antibody...Ch. 19 - 15. Describe the functions of the RAG1 and RAG2...Ch. 19 - According to the scenario shown in Figure 20.7,...Ch. 19 - Prob. 17CONQCh. 19 - Prob. 18CONQCh. 19 - Prob. 19CONQCh. 19 - 19. Why does transposition always produce direct...Ch. 19 - 20. Which types of TEs have the greatest potential...Ch. 19 - Prob. 22CONQCh. 19 - 22. Let’s suppose that a species of mosquito has...Ch. 19 - This chapter describes different types of TEs,...Ch. 19 - Prob. 25CONQCh. 19 - Prob. 29CONQCh. 19 - 26. What is the difference between an autonomous...Ch. 19 - Prob. 26CONQCh. 19 - Prob. 28CONQCh. 19 - 1. Briefly explain how McClintock determined that...Ch. 19 - Prob. 2EQCh. 19 - The work of McClintock showed that the presence of...Ch. 19 - 3. In your own words, explain the term transposon...Ch. 19 - Prob. 5EQCh. 19 - 5. Gerald Rubin and Allan Spradling devised a...Ch. 19 - Make a list of the similarities and differences...Ch. 19 - Prob. 2QSDCCh. 19 - Prob. 3QSDC
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- Host DNA is digested into small pieces, which are occasionally assembled with phage proteins, creating a phage with bacterial chromosomal DNA. If the breakage of the chromosomal DNA is not random (i.e., it is more likely tobreak at certain spots as opposed to other spots), how might suchnonrandom breakage affect cotransduction frequency?arrow_forwardWhich of the following statements describes the lysogenic cycle of lambda (A) phage? After infection, the viral genes immediately turn the host cell into a lambda-producing factory, and the host cell then lyses. Most of the prophage genes are activated by the product of a particular prophage gene. The phage DNA is incorporated into the bacteria DNA The phage DNA is copied and exits the cell as a phage. The phage DNA get destructed by the host bacteria DNAarrow_forwardDescribe the reason behind the occurrence of phage growth and release by the mating with the non-lysogenic recipient, but not with the infection by a lysogenic recipient.arrow_forward
- Indicate whether each statement applies to the lytic or lysogenic cycle of phage A, or to both. 1. The host cell is lysed. (Click to select) v 2. The genetic material of the phage is copied. (Click to select) v 3. The genetic material of the phage is integrated into the bacterium's chromosome. (Click to select) v 4. Two ends of the phage DNA become covalently attached to one another. (Click to select) v 5. The integrase gene is turned on.: (Click to select) v (Click to select) lytic lysogenic botharrow_forwardWhen bacteriophage P1 causes E. coli to lyse, the resulting materialis called a P1 lysate. What type of genetic material would befound in most of the P1 phages in the lysate? What kind of geneticmaterial is occasionally found within a P1 phage?arrow_forwardThis gene is responsible for making the protein that catalyzes the recombination of the phage genome into the host bacterial chromosome is: excise integrase attP attBarrow_forward
- When a temperate phage infects a bacteria cell it O immediately undergoes the temperate lifecycle. O immediately integrates its genome into the host cell genome; when conditions are harsh, the phage excises out of the host chromosome and undergoes the lytic lifecycle. O immediately undergoes the lytic lifecycle. O can either immediately undergo the lytic lifecycle or immediately undergo the lysogenic lifecycle, and it usually undergoes the lytic lifecycle.arrow_forwardMost phages that use DNA to propagate work by hijacking host bacterial machinery including RNA Polymerase. Scientists found that treatment of jumbo bacteriophage PhiKZ with Rifampicin did not stop the phage from propagating. What is allowing for the survival of this phage?arrow_forwardCompare and contrast the lysogenic and lytic cycles of phage λ.arrow_forward
- A phage that reproduces slowly, produces relatively few phage particles, and only undergoes the lytic life cycle most likely will produce a plaque that is: OSmaller and turbid/hazy Smaller and clear Larger and turbid/hazy Larger and cleararrow_forwardWhy is a webbed plate capable of producing a higher lysate titer (pfu/mL) than a cleared plate? a.) A webbed plate has plaques that have gone through more rounds of replication because the number of host cells did not limit phage replication, creating exponentially more phage particles b.) A webbed plate has no more host available for replication, meaning the plate is full of exponentially higher amounts of phage c.) A cleared plate has less bacteria than a webbed plate, so there are more plaques present d.) A cleared plate contains more phage than a webbed plate because it started with a more concentrated phage samplearrow_forwardA phage that reproduces slowly, produces relatively few phage particles, and only undergoes the lytic life cycle most likely will produce a plaque that is: Smaller and clear Smaller and turbid/hazy Larger and turbid/hazy Larger and cleararrow_forward
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