Vander's Human Physiology
15th Edition
ISBN: 9781259903885
Author: WIDMAIER, Eric P., Vander, Arthur J.
Publisher: Mcgraw-hill Education,
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Chapter 18, Problem 6PI
Summary Introduction
To determine: The advantage of having B cells that differentiate into memory cells.
Introduction: B cells or the B lymphocytes are a type of leucocyte that plays a major role in humoral immunity by secreting antibodies. They are also involved in secretion of several cytokines and act as antigen-presenting cells. The cells are produced and matured in the bone marrow. The B cells have special receptors on their surface known as B cell receptors (BCR) that make them specific for each antigen.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 18 Solutions
Vander's Human Physiology
Ch. 18 - Prob. 1PICh. 18 - Prob. 2PICh. 18 - Prob. 3PICh. 18 - Into which types of cells do myeloid stem cells...Ch. 18 - Prob. 5PICh. 18 - Prob. 6PICh. 18 - Prob. 7PICh. 18 - Prob. 8PICh. 18 - What are the major cells of the immune system and...Ch. 18 - Describe the major anatomical and biochemical...
Ch. 18 - Name the three cell types that function as...Ch. 18 - List the sequence of events in an inflammatory...Ch. 18 - Name the sources of the major inflammatory...Ch. 18 - What triggers the alternative pathway for...Ch. 18 - Prob. 7RQCh. 18 - Name the lymphoid organs. Contrast the functions...Ch. 18 - Name the various populations and subpopulations of...Ch. 18 - Prob. 10RQCh. 18 - Prob. 11RQCh. 18 - Prob. 12RQCh. 18 - Prob. 13RQCh. 18 - Prob. 14RQCh. 18 - What two processes contribute to immune...Ch. 18 - Prob. 16RQCh. 18 - Prob. 17RQCh. 18 - Prob. 18RQCh. 18 - Prob. 19RQCh. 18 - Prob. 20RQCh. 18 - Prob. 21RQCh. 18 - Prob. 22RQCh. 18 - Prob. 23RQCh. 18 - Prob. 24RQCh. 18 - Prob. 25RQCh. 18 - Prob. 26RQCh. 18 - Prob. 27RQCh. 18 - Prob. 1RRCh. 18 - Prob. 2RRCh. 18 - Prob. 1RCCh. 18 - Prob. 2RCCh. 18 - Prob. 3RCCh. 18 - Prob. 4RCCh. 18 - Prob. 5RCCh. 18 - Prob. 6RCCh. 18 - Prob. 7RCCh. 18 - Prob. 8RCCh. 18 - Prob. 9RCCh. 18 - Prob. 10RCCh. 18 - Prob. 1AAECh. 18 - Prob. 2AAECh. 18 - Prob. 3AAECh. 18 - Prob. 4AAECh. 18 - Prob. 5AAECh. 18 - Homeostasis is essential for health and survival....
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