The way in which a strain of yeast is used to identify the prey proteins along with the ways in which this protocol is relevant to the goal of finding the proteins. Introduction: The yeast two-hybrid system method has three components. First: the yeast that contains a reporter gene construct in which UASG drives the expression of an E.Coli . Second: the yeast expresses the fusion protein in which the DNA binding domain of GAL4 is fused to the protein of interest. Third: a cDNA library made in plasmids where each cDNA is fused in frame to the activation domain of GAL4.

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Chapter 17, Problem 17P

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To determine:

The way in which a strain of yeast is used to identify the prey proteins along with the ways in which this protocol is relevant to the goal of finding the proteins.

Introduction:

The yeast two-hybrid system method has three components. First: the yeast that contains a reporter gene construct in which UASG drives the expression of an E.Coli. Second: the yeast expresses the fusion protein in which the DNA binding domain of GAL4 is fused to the protein of interest. Third: a cDNA library made in plasmids where each cDNA is fused in frame to the activation domain of GAL4.

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