Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 10, Problem 25P
A second strain of dwarf plants has a different mutation of the same gene identified in Problem
What mutational mechanism is most likely responsible for the production of abnormal DNA fragment length corresponding to this mutant allele? Explain your reasoning.
In comparison to the length of mRNA from the normal allele, will the mRNA from this mutant allele most likely be longer, shorter, or about the same length? Explain your answer.
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QUESTION 11
The location of five deletions have been mapped to a Drosophila chromosome, as shown in the following deletion map.
Recessive mutations a, b, c, d and e are known to be located in the same region as the deletions but the order of the
mutations on the chromosome is not known.
Drosophila Chromosome
Deletion 1
Deletion 2
Deletion 3
Deletion 4
Deletion 5
When flies homozygous for the recessive mutation are crossed with flies heterozygous for the deletions, the following
results are obtained in which "m" represents recovery of flies with a mutant phenotype and "+" represents recovery of
only flies with a wildtype phenotype. Use the data from the table below to determine the order of the genes (a-e) on the
chromosome.
Mutants
Deletions
a
b
C
d
e
1
+
+
+
+
m
2
+
m
+
m
3
m
m
m
m
4
+
+
m
+
+
5
m
+
m
m
+
What is the gene order for these five genes? Please do not separate the letters representing the mutations with
spaces or punctuation (e.g. LMNOP)
Transgenic tobacco plants were obtained in which the vector Ti plasmid was designed to insert the gene of interest plus an adjacent kanamycin-resistance gene. The inheritance of chromosomal insertion was followed by testing progeny for kanamycin resistance. Two plants typified the results obtained generally. When plant 1 was backcrossed with wild-type tobacco, 50 percent of the progeny were kanamycin resistant and 50 percent were sensitive. When plant 2 was backcrossed with the wild type, 75 percent of the progeny were kanamycin resistant and 25 percent were sensitive. What must have been the difference between the two transgenic plants? What would you predict about the situation regarding the gene of interest?
3) You have identified an interesting mutant in gene P. Using a Punnett square, demonstrate the cross you perform to determine if it is a dominant or recessive mutation compared to the WT gene P allele. Write the expected ratios for either scenario.
4) You have determined the mutation is dominant when compared to the WT allele. Briefly describe a technique you could use to determine if expression levels of gene P have been altered in this mutant.
5) You have determined the expression level of gene P has increased. What class/type of mutation would cause this?
Chapter 10 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 10 - Define the following terms as described in this...Ch. 10 - 2. Using sickle cell disease as an example,...Ch. 10 -
3. Compare and contrast the contributions of...Ch. 10 - Why do differences in protein electrophoretic...Ch. 10 - Prob. 5PCh. 10 - Prob. 6PCh. 10 - Prob. 7PCh. 10 - 8. Wildtype βglobin protein is composed of amino...Ch. 10 - Prob. 9PCh. 10 - Prob. 10P
Ch. 10 - 11. How is an autoradiograph produced from a...Ch. 10 - Prob. 12PCh. 10 - Prob. 13PCh. 10 - Prob. 14PCh. 10 - The family represented in the pedigree and...Ch. 10 - Suppose the mating couple (I-1 and I-2) shown in...Ch. 10 - What are restriction endonucleases, and why are...Ch. 10 - 18. Following restriction digestion, DNA fragments...Ch. 10 - 19. The doublestranded DNA sequence below is part...Ch. 10 - 20. Restriction enzymes recognize specific...Ch. 10 - Prob. 21PCh. 10 - Prob. 22PCh. 10 - Prob. 23PCh. 10 - Prob. 24PCh. 10 - 25. A second strain of dwarf plants has a...Ch. 10 - During gel electrophoresis of linear DNA...Ch. 10 - Prob. 27PCh. 10 - 28. In molecular biology, restriction...Ch. 10 - A complete plant gene containing four introns and...Ch. 10 - Prob. 30PCh. 10 - The map below illustrates three alleles in a...Ch. 10 - Prob. 32PCh. 10 - 33. Northern blot analysis is performed on mRNA...
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