
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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- You will prepare 5 cuvettes with different concentration of sodium phosphate (Pi) using the stock 1mM Pi, 0.5mM PNPP solution (in water) and 0.2M Tris-HCl, pH=8.00. For this part, the concentration of PNPP will remain constant at 0.05mM. Fill the table below (before coming to lab) and use it to prepare your cuvettes. Your blank for each reaction is the PNPP mixed with Tris-HCL and Pi. DO NOT ADD ENZYME AT THIS POINT!!!
Final [Pi] uM |
Volume of 1mM Pi |
Final [PNPP] mM |
Volume of 0.5mM PNPP |
Volume of 0.2M Tris-HCl (uL) |
Volume of enzyme (uL) |
Total Volume (uL) |
0 |
0.05 |
100 |
1500 |
|||
3.33 |
0.05 |
100 |
1500 |
|||
20 |
0.05 |
100 |
1500 |
|||
50 |
0.05 |
100 |
1500 |
|||
100 |
0.05 |
100 |
1500 |
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