You will prepare 5 cuvettes with different concentration of sodium phosphate (Pi) using the stock 1mM Pi, 0.5mM PNPP solution (in water) and 0.2M Tris-HCl, pH=8.00. For this part, the concentration of PNPP will remain constant at 0.05mM. Fill the table below (before coming to lab) and use it to prepare your cuvettes. Your blank for each reaction is the PNPP mixed with Tris-HCL and Pi. DO NOT ADD ENZYME AT THIS POINT!!! Final [Pi] uM Volume of 1mM Pi Final [PNPP] mM Volume of 0.5mM PNPP Volume of 0.2M Tris-HCl (uL) Volume of enzyme (uL) Total Volume (uL) 0   0.05     100 1500 3.33   0.05     100 1500 20   0.05     100 1500 50   0.05     100 1500 100   0.05     100 1500

Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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  • You will prepare 5 cuvettes with different concentration of sodium phosphate (Pi) using the stock 1mM Pi, 0.5mM PNPP solution (in water) and 0.2M Tris-HCl, pH=8.00. For this part, the concentration of PNPP will remain constant at 0.05mM. Fill the table below (before coming to lab) and use it to prepare your cuvettes. Your blank for each reaction is the PNPP mixed with Tris-HCL and Pi. DO NOT ADD ENZYME AT THIS POINT!!!

Final [Pi] uM

Volume of 1mM Pi

Final [PNPP] mM

Volume of 0.5mM PNPP

Volume of 0.2M Tris-HCl (uL)

Volume of enzyme (uL)

Total Volume (uL)

0

 

0.05

   

100

1500

3.33

 

0.05

   

100

1500

20

 

0.05

   

100

1500

50

 

0.05

   

100

1500

100

 

0.05

   

100

1500

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