You prepare several dilutions of an unknown compound. You measure the absorbance of each solution at 340 nm using a 1 cm cuvette (your results are listed in the table below). What is the extinction coefficient (in (Mecm) ') of the compound? (Hint: assume that each of the individual values contains some degree of experimental error.) Concentration(uM) Absorbance at 340 nm 10.0 0.011 20.0 0.023 40.0 0.066 80.0 0.119 160.0 0.189

A standard containing 20 mg/mL of compound P is placed in a 1 cm cuvette. The absorbance of the standard is 1.20 at 600 nm.  A sample containing Compound P has an absorbance of 0.50.  What is the concentration of this sample?

What is the molar extinction coefficient at 625 nm for this copper (II) acetate solution? How was it determined? Standard Solution Concentration (M) Absorbance 1 0.025 0.049 2 0.050 0.153 3 0.100 0.242 4 0.200 0.499 5 0.250 0.694

tab caps lock You have a 11.5 mg sample of blood that contains various proteins. Hemoglobin is the only protein in the sample containing Fe. Hemoglobin contains 3.83% by mass of a compound called heme (C34H32FeN4O4, MW = 616.49 g/mol). You find that your 11.5 mg blood sample contains 29.7 micrograms of Fe. What is the mass percent of hemoglobin in your protein sample? Tap here or pull up for additional resources Q A NO 2 W S #3 E D GA 4 R % 5 F Question 9 of 17 T 6 G Y & 7 B H 8 N 9 4 +/ V M

How would I do 2? the concentration iron is 1.85mg/vitamin tablet, and the absorbance at 508nm is 0.37

A colored ion solution has a concentration of 0.200 M with a measured absorbance A = 0.880. Another ion solution made of the same chemicals has an absorbance A = 0.172. What is the concentration of this unknown sample solution?

There is a substance in a solution (8 g/liter). The length of cuvette is 2 cm and the extinction coefficient is 0.0376 M-1 cm-1, how much is the beam of light is transmitted?

From the following data, calculate the concentration of the analyte in the sample read at 700 nm: Absorbance of unknown sample = 0.807 Absorbance of a 130 mg/dl standard = 0.234 Do not answer in image format. Maintain accuracy and quality in your answer. Answer completely.

2. ] You make 150.0 mL of a copper(II) sulfate solution but forget to cover it before leaving for the day. The next class, you measure the volume and find that you only have 132.0 mL remaining. You didn’t record the initial concentration, but you measure the absorbance of the remaining solution at 620 nm and find that it is 0.386. You also construct the following calibration curve for CuSO4: Standard solution Abs620 0.50 M 0.424 0.40 M 0.336 0.30 M 0.247 0.20 M 0.159 (refer to image) a. What is the value for k (in Beer’s Law)? Provide a calculation or graph to support your answer. b. What is the concentration of the (remaining) solution? c. What was the concentration of the original solution from the first class? d. If you removed 7.5 mL from the 132.0 mL solution in the process of measuring the absorbance, how much water should you add to obtain the original concentration that you solved for in part (c)?

A medical lab technician creates a standard solutions of percent hemoglobin in whole blood. The technician measures the absorbance of each solution at 520 nm and creates a standard curve of the absorbance versus percent hemoglobin in whole blood and finds the linear equation below, with an R2 of 0.9985.                                                  y= 0.0523x + 0.072 A patient sample has an absorbance of 0.893. Using the standard curve data, calculate the percent hemoglobin in the patient sample.