You plated 15,000 cells on a T25 to make a happy well. How many cells do you need to plate in a 6 well plate? A 24 well plate?

The diagrams below show the results of three FRAP experiments. Match the proteins to the statements. Protein B 1.0 0.8- 0.6- EVL 0.4- 0.2- 50 100 150 time (seconds) 0.2- 50 Protein A 100 150 time (seconds) 200 250 Protein that is completely tethered/anchored and does not move in the memebrane Protein that has some of it moving slowly while others are tethered/anchored in the membrane Protein that is fully mobile in the membrane Represent proteins associated with the cell membrane Represent cytoplasmic proteins that are moving around [Choose ] [Choose ] A, B and C None of these A Both A and B B с [Choose ] [Choose ] [Choose ] 200 250 50 Protein C 100 150 time (seconds) T 200 250

Which staining method was used in the picture? O Nissl staining O In situ hybridization Silver impregnation (the Golgi method) Immunostaining with an antibody O Intracellular injection of a fluorescent dye

Q5

If a percent difference is a positive number, what does this tell us about the change in weight of the artificial cell?

Compared to cells with a SA: V ratio of 2.1, cells with a SA:V ratio of 1.3 would be expected to Group of answer choices lack a cell membrane have a single, circular chromosome be found only in multicellular organisms grow and divide more slowly be able to obtain oxygen for their cellular reactions more readily

You counted 4, 6, 12, 3 cells in each of the 4.outed squares of a hemacytomeyer. What are the cells per milliliter in that culture? If you resuspended your cell pellet 2.5 mL, what is the total cell count? How many uL do you need to add to a new culture if you want 4250 cells?

Give typing answer with explanation and conclusion

Your HepG2 cells have reached 80 % confluency. What should you do next? Explain the procedures in detail.

(Select all that are correct) The external force applied on cells could potentially induce the cell to produce different proteins excert forces differently on its external microenvironment move Ochange shape die