You have two DNA samples, A and B. A 1/10 dilution of sample A had an absorbance at 260nm of 0.12. A 1/25 dilution of sample B had an absorbance at 260nm of 0.12. 5µL of the undiluted sample A and 5uL of the undiluted sample B were added to 90 µl of water. What is the final DNA concentration in ug/µL?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You have two DNA samples, A and B. A 1/10
dilution of sample A had an absorbance at
260nm of 0.12. A 1/25 dilution of sample B had
an absorbance at 260nm of 0.12. 5µL of the
undiluted sample A and 5µL of the undiluted
sample B were added to 90 µL of water. What is
the final DNA concentration in µg/µL?
Transcribed Image Text:You have two DNA samples, A and B. A 1/10 dilution of sample A had an absorbance at 260nm of 0.12. A 1/25 dilution of sample B had an absorbance at 260nm of 0.12. 5µL of the undiluted sample A and 5µL of the undiluted sample B were added to 90 µL of water. What is the final DNA concentration in µg/µL?
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