You have purified a new peptide hormone. To determine its amino acid sequence you have digested the polypeptide with trypsin and in a separate reaction you have cleaved the polypeptide with cyanogen bromide. Cleavage with trypsin yielded 5 peptides that were sequenced by Edman degradation as shown in the following. 1. Ser-Leu 2. Asp-Val-Arg 3. Val-Met-Glu-Lys 4. Ser-Gln-Met-His-Lys 5. Ile-Phe-Met-Leu-Cys-Arg Cleavage with cyanogen bromide yielded 4 peptides that were sequenced by Edman degradation: 1. His-Lys-Ser-Leu 2. Asp-Val-Arg-Val-Met 3. Glu-Lys-lle-Phe-Met 4. Leu-Cys-Arg-Ser-Gln-Met Determine the identity of the N-terminal amino acid after reconstructing the intact protein. Glu O lle Ser Asp ○ His
You have purified a new peptide hormone. To determine its amino acid sequence you have digested the polypeptide with trypsin and in a separate reaction you have cleaved the polypeptide with cyanogen bromide. Cleavage with trypsin yielded 5 peptides that were sequenced by Edman degradation as shown in the following. 1. Ser-Leu 2. Asp-Val-Arg 3. Val-Met-Glu-Lys 4. Ser-Gln-Met-His-Lys 5. Ile-Phe-Met-Leu-Cys-Arg Cleavage with cyanogen bromide yielded 4 peptides that were sequenced by Edman degradation: 1. His-Lys-Ser-Leu 2. Asp-Val-Arg-Val-Met 3. Glu-Lys-lle-Phe-Met 4. Leu-Cys-Arg-Ser-Gln-Met Determine the identity of the N-terminal amino acid after reconstructing the intact protein. Glu O lle Ser Asp ○ His
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter9: Gene Expression And Gene Regulation
Section: Chapter Questions
Problem 18QP: The following is a portion of a protein: met-trp-tyr-arg-gly-pro-thr-Various mutant forms of this...
Related questions
Question
help #14
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 2 steps
Recommended textbooks for you
Human Heredity: Principles and Issues (MindTap Co…
Biology
ISBN:
9781305251052
Author:
Michael Cummings
Publisher:
Cengage Learning
Human Heredity: Principles and Issues (MindTap Co…
Biology
ISBN:
9781305251052
Author:
Michael Cummings
Publisher:
Cengage Learning