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You have purified a new peptide hormone. To determine its amino acid sequence you have digested the polypeptide with trypsin and in a separate reaction you have cleaved the polypeptide with cyanogen bromide. Cleavage with trypsin yielded 5 peptides that were sequenced by Edman degradation as shown in the following. 1. Ser-Leu 2. Asp-Val-Arg 3. Val-Met-Glu-Lys 4. Ser-Gln-Met-His-Lys 5. Ile-Phe-Met-Leu-Cys-Arg Cleavage with cyanogen bromide yielded 4 peptides that were sequenced by Edman degradation: 1. His-Lys-Ser-Leu 2. Asp-Val-Arg-Val-Met 3. Glu-Lys-lle-Phe-Met 4. Leu-Cys-Arg-Ser-Gln-Met Determine the identity of the N-terminal amino acid after reconstructing the intact protein. Glu O lle Ser Asp ○ His

You have purified a new peptide hormone. To determine its amino acid sequence you have digested the polypeptide with trypsin and in a separate reaction you have cleaved the polypeptide with cyanogen bromide. Cleavage with trypsin yielded 5 peptides that were sequenced by Edman degradation as shown in the following. 1. Ser-Leu 2. Asp-Val-Arg 3. Val-Met-Glu-Lys 4. Ser-Gln-Met-His-Lys 5. Ile-Phe-Met-Leu-Cys-Arg Cleavage with cyanogen bromide yielded 4 peptides that were sequenced by Edman degradation: 1. His-Lys-Ser-Leu 2. Asp-Val-Arg-Val-Met 3. Glu-Lys-lle-Phe-Met 4. Leu-Cys-Arg-Ser-Gln-Met Determine the identity of the N-terminal amino acid after reconstructing the intact protein. Glu O lle Ser Asp ○ His

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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You have purified a new peptide hormone. To determine its amino acid sequence you have digested the polypeptide with trypsin and in a separate reaction you have cleaved the polypeptide with cyanogen bromide. Cleavage with trypsin yielded 5 peptides that were sequenced by Edman degradation as shown in the following. 1. Ser-Leu 2. Asp-Val-Arg 3. Val-Met-Glu-Lys 4. Ser-Gln-Met-His-Lys 5. Ile-Phe-Met-Leu-Cys-Arg Cleavage with cyanogen bromide yielded 4 peptides that were sequenced by Edman degradation: 1. His-Lys-Ser-Leu 2. Asp-Val-Arg-Val-Met 3. Glu-Lys-lle-Phe-Met 4. Leu-Cys-Arg-Ser-Gln-Met Determine the identity of the N-terminal amino acid after reconstructing the intact protein. Glu O lle Ser Asp ○ His
Transcribed Image Text:You have purified a new peptide hormone. To determine its amino acid sequence you have digested the polypeptide with trypsin and in a separate reaction you have cleaved the polypeptide with cyanogen bromide. Cleavage with trypsin yielded 5 peptides that were sequenced by Edman degradation as shown in the following. 1. Ser-Leu 2. Asp-Val-Arg 3. Val-Met-Glu-Lys 4. Ser-Gln-Met-His-Lys 5. Ile-Phe-Met-Leu-Cys-Arg Cleavage with cyanogen bromide yielded 4 peptides that were sequenced by Edman degradation: 1. His-Lys-Ser-Leu 2. Asp-Val-Arg-Val-Met 3. Glu-Lys-lle-Phe-Met 4. Leu-Cys-Arg-Ser-Gln-Met Determine the identity of the N-terminal amino acid after reconstructing the intact protein. Glu O lle Ser Asp ○ His
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