Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Pleaseeeeeeeee explain the processs of this I’m so confuseddddddd………….
You cleave a peptide with chymotrypsin and sequence the resulting peptides and get the following: Met-Arg-Ala-Tyr Asp-Met-Leu-Phe Gly-Asn You cleave the same peptide as the one digested with chymotrypsin above with cyanogen bromide. Following sequencing, you get the resulting peptides Arg-Ala-Tyr-Gly-Asn Leu-Phe-Met Asp-Met
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Transcribed Image Text:You cleave a peptide with chymotrypsin and sequence the resulting peptides and get the following: Met-Arg-Ala-Tyr Asp-Met-Leu-Phe Gly-Asn You cleave the same peptide as the one digested with chymotrypsin above with cyanogen bromide. Following sequencing, you get the resulting peptides Arg-Ala-Tyr-Gly-Asn Leu-Phe-Met Asp-Met
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