You are to make 250mL of Edward’s Buffer, the recipe of which is as follows: 25mM EDTA, 250mM Sodium Chloride, 0.5% SDS, and 200mM Tris. But you only have the stock solutions of the following concentration: 150mM EDTA, 2M Sodium Chloride, 20% SDS, and 1M Tris. Explain what each result means in a brief sentence, and indicate how much water is needed.
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You are to make 250mL of Edward’s Buffer, the recipe of which is as follows:
25mM EDTA, 250mM Sodium Chloride, 0.5% SDS, and 200mM Tris.
But you only have the stock solutions of the following concentration:
150mM EDTA, 2M Sodium Chloride, 20% SDS, and 1M Tris.
Explain what each result means in a brief sentence, and indicate how much water is needed.
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