You are now the chemist responsible for describing the efficiency of a newly produced GC column that is 25 meters long. You do this by injecting an aliquot of an octadecane standard into the column and observing its resultant chromatographic output. What type of chromatography are you performing in this experiment? (What would you assume the polarity of the mobile and stationary phases are in this efficiency experiment? Why? (s) You find that the retention time for the standard is 8.22 minutes and the width at half height is 0.047 minutes. The chromatographic peak shape is Guassian. Calculate the number of theoretical plates per length (m) of column in this column. (

You are now the chemist responsible for describing the efficiency of a newly produced GC column that is 25 meters long. You do this by injecting an aliquot of an octadecane standard into the column and observing its resultant chromatographic output. What type of chromatography are you performing in this experiment? (What would you assume the polarity of the mobile and stationary phases are in this efficiency experiment? Why? (s) You find that the retention time for the standard is 8.22 minutes and the width at half height is 0.047 minutes. The chromatographic peak shape is Guassian. Calculate the number of theoretical plates per length (m) of column in this column. (

EBK A SMALL SCALE APPROACH TO ORGANIC L

4th Edition

ISBN:9781305446021

Author:Lampman

Publisher:Lampman

Chapter88: Column Chromatography

Section: Chapter Questions

Problem 3P

See similar textbooks

Similar questions

1. From spinach, the chromatographic analysis will yield two major substances: (a) B-carotene (C40H56), which will separate from the spinach mixture to form a yellow band, and (b) chlorophyll A (CSSH72MGN4OS), which will separate from the mixture to form a green band. i. Describe the characteristics and draw structures of these molecules. ii. From the spectrophotometric analysis, what wavelength (Amax) would you expect ß-carotene (C40H56) to absorb? What wavelength would chlorophyll A (CssH72MgN4O5) absorb?
You are carrying out an experiment by analysing alcoholic drinks by gas chromatography. Why is Gas chromatography the best technique used for this analysis? Why is Carbowax a suitable stationary phase for this analysis? What gases are used and what is their purpose and source?
True or False: Liquid-solid phase column chromatography is used to separate large up to multigram amounts of mixtures, based on their differences (however small) in polarity.
What are the disadvantages of uses the Thick layer stationary phase in Thin layer Chromatography (TLC)? Please shortly answer at your own words. Answer should be to the point ( specific 4-5 lines).
One method for the quantitative determination of the concentration of constituents in a sample analyzed by gas chromatography is area normalization. Here, complete elution of all the sample constituents is necessary. The area of each peak is then measured and corrected for differences in detector response to the different eluates. This correction involves dividing the area by an empirically determined correction factor. The concentration of the analyte is found from the ratio of its corrected area to the total corrected area of all peaks. For a chromatogram containing three peaks, the relative areas were found to be 16.4, 45.2 and 30.2, in order of increasing retention time. Calculate the percentage of each compound if the relative detector responses were 0.60, 0.78 and 0.88, respectively.
In color chromatography of plant pigments, what complications would a dried-out column (solvent level is below the top of the silica) introduce to the elution and isolation of pigments?
One method for quantitative determination of the concentration of constituents in a sample analyzed by gas chromatography is the area normalization method. In this method, complete elution of all of the sample constituents is necessary. The area of each peak is then measured and corrected for differences in detector response to the different eluates. This correction is accomplished by dividing the area by an empirically determined correction factor. The concentration of the analyte is found from the ratio of its corrected area to the total corrected area of all peaks. For a chromatogram containing three peaks, the relative areas were found to be 16.4, 45.2, and 30.2 in the order of increasing retention time. Calculate the percentage of each compound if the relative detector responses were 0.60, 0.78, and 0.88, respectively
Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the demonstration in TLC and paper chromatography? How do you say so?
Cite the applications for utilizing molecular/size exclusion chromatography in the analysis of samples. What are some of the limitations when using this analytical method?