You are given the suggestion that a 0.15 ppm quinine aqueous solution is a good concentration to shoot for in the experiments, but quinine fluoresces best when it is acidified to 0.10 M H2SO4. Describe how to make 100 mL of the recommended solution starting with quinine sulfate (actually, quinine hemisulfate salt monohydrate). Assume you also have 1.0 M sulfuric acid and de-ionized water. Describe the process of a substance undergoing fluorescence and compare to the process of phosphorescence.

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Applications of Molecular Spectroscopy

When light sources pass through the diffraction grating then light splits into different wavelengths. The light source produces two types of patterns that are shown as destructive and non-destructive. The light source is in the form of quanta that is quantitation.