With respect to the Multiple Sequence Alignment software tool, ClustalW, briefly give an overview of the 3 steps involved in generating a Multiple Sequence Alignment

Q: 27. Based on your knowledge of the structure and organization of prokaryotic and eukaryotic gen…

A: Prokaryotes are primitive cells which have simple cellular organisation. They do not have any…

Q: reproductive cloning

A: Apart from the natural reproduction of various organisms in our ecosystem, the advent of technology…

Q: 1. For this gene (I marked the +1 site) a. Identify the promoter, template, and coding strand b.…

A: Promoter is a region on the DNA where RNA polymerase enzyme binds for the process of transcription.…

Q: 49 Identify which PCR primer that could be used to amplify the gene shown (choose all correct…

A: Since there are multiple questions in this particular question, I will answer the first one for you.…

Q: 4. 16B (Eukaryotes): Describe 4 factors that differentiate eukaryotic chromosomes/genome from a…

A: Nucleus is a chief controller of the cell which carries genetic instructions . It comprises of…

Q: 12. a. You want to create a genetic construct that will express GFP in Drosophila. In addition to…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Explain how the SNP changes the recognition sequence for the restriction enzyme and affects its…

A: *Single nucleotide polymorphisms (SNPs) involve variation of a single base pair and or SNPsbin the…

Q: Briefly describe two approaches that are used to edit the germline in embryos using CRISPR-Cas9.…

A: The mechanism of CRISPR/Cas-9 genome editing can be generally divided into three steps: recognition,…

Q: 6 7 8 Chromosome 9 10 11 12 13 14 15 16 17 18 19202122 a) From the plot above. Which chromosome/s…

A: Genome refers to the complete set of genes or the complete genetic makeup of an individual organism.…

Q: a. What sequence information about a gene is lackingin a cDNA library?b. Can clones in a cDNA…

A: The cDNA or complementary DNA is derived from mRNA which is transcribed from genomic DNA (gDNA).  It…

Q: Briefly discuss (using three sentences) how the concepts and/or techniques in molecular biology are…

A: Introduction Molecular biology:- It is the branch of biology that studies the molecular basis of…

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A: Introduction Recombinant DNA technology or RDT is a process by which the genetic material of an…

Q: using illustrations describe the structure of a typical cloning vector and discuss the functions of…

A: Vector is a DNA molecule that is used as a carrier or vehicle for carrying foreign genetic material…

Q: Describe step 1: hybridization of template and primer in automated sanger sequencing

A: Introduction  Sanger sequencing is also known as the “chain termination method”. It is a method for…

Q: List other methods of reverse genetics available in S. cerevisiae, Drosophila, Arabidopsis, and…

A: Saccharomyces cerevisiae commonly known as the yeast  are micro organism with application in…

Q: Write advantage and drawbacks of RISA (Ribosomal Intergenic Spacer Analysis) technique. What is the…

A: RISA is the technique for microbial community analysis. It helps in determining the species…

Q: list and explain two strategic between the shotgun sequences/ assemble strategy for whole genome…

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Q: In Figure 10-10, determine approximately how manyBAC clones are needed to provide 1× coverage ofa.…

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Q: Animation 12.1: This table shows results from allele specific oligonucleotide hybridization analyses…

A: Blotting techniques are the methods used in the molecular biology for the identification or…

Q: Discuss the bioinformatics approaches that can be used to identifya protein-encoding gene.

A: The process of recognizing the protein-encoding gene is crucial and has numerous applications. Such…

Q: Exaplain the two methods to study the contents of transcriptomes which are microarray analysis and…

A: Transcriptome analysis may be used to find novel genes and isoforms that are not already cataloged…

Q: 11) Draw a bacterial expression vector with all required vector sequences. Be sure to label any…

A: Bacterial expression vectors are used to introduce foreign genetic material into a host in order to…

Q: 24. The diagram below shows the map for EcoRI restriction sites around the BRCA1 gene for two…

A: Answer well explained above.

Q: Explain how DNA probes with different fluorescence emissionwavelengths can be used in a single FISH…

A: The FISH (fluorescence in situ hybridization) experiment uses a probe that is fluorescently labeled…

Q: The xyz gene product, encoded by a new viral strain, has corresponding human homologs. Blast…

A: Substitution matrix is used to sequence the aligned position. The substitution matrix is of two…

Q: 33) Judging by mosaic clone images above, what effect does loss of dmyc likely have on cell growth?…

A: Approach to solving the question:1. Observation of Mosaic Clone Images: Begin by carefully examining…

Q: Genetic linkage studies can usually locate the chromosomal position of a disease gene only roughly.…

A: The chromosomal theory of inheritance provides the idea that genes are located on the chromosomes.…

Q: Below is shown an 8kb region of the human genome, with the proportion of the nucleotides that are…

A: Introduction :  Any segment of a gene that will be included in the mature RNA produced by that gene…

Q: 1. Using the data available describe the phenotype that you observe when each of these three genes…

A: The question is related to the use of RNA interference (RNAi) to study the function of specific…

Q: Determine the size of the DNA you would expect from the restriction digests of the GABRD and GABRD…

A: Gel electrophoresis is a technique used to separate DNA, RNA, or protein molecules based on their…

Q: What are potential uses of CRISPR-Cas9 Technologies? Silence genes to study their function Identify…

A: CRISPR-Cas9 is a gene editing tool that allows scientists to precisely target and cut specific DNA…

Q: 1) Enhanced GFP protein has: An excitation peak at 488nm and emits maximally at 507nm. An excitation…

A: Answer 1.  Answer. 1). Enhanced GFP protein has an excitation peak at 488nm and emits maximally…

Q: . Below are the DNA sequences that encode the first eight amino acids for five alleles of the Adh…

A: DNA (deoxyribonucleic acid) is the genetic material present in most organisms. The segment of DNA…

Q: 7. Consider the 3 mechanisms of HGT in prokaryotes and identify 1) which one leads to the presence…

A: Horizontal gene transfer (HGT) refers to the transfer of genetic material from one species to…

Q: 1a.The complete genotype of the host yeast strain is MATa ade2 his3 leu2 met15 ura3.  Name one…

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Q: describe how DNA shuffling and molecular breeding are used to generate hybrid genes and the…

A: DNA shuffling or molecular breeding is an in-vitro molecular method, where mutant genes are created…

Q: . Where is this gene location in the bacterial genome? . What is the length of the gene? . Obtain…

A: Salmonella is usually found in the Gastrointestinal tracts of mammals

Q: 9.) Describe the molecular mechanisms of mutagenesis. Consider Inciuding the bases impacted, the…

A: Mutagenesis is the process by which the genetic information of an organism gets changed by mutation.…

Q: Discuss the reasons why the proteome is larger than the genome ofa given species.

A: The genome of an individual is referred to as the organism's DNA with all the genes. It includes…

Q: Check all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing…

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Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.