thank you!!! Why is the temperature inside the thermal cycler lowered to 55°C during the PCR process?Select one:O a. To slow down the reaction halfway throughO b. To speed up the reaction halfway throughO c. To allow primers to bind to single-stranded DNAO d. To enhance the action of the Taq Polymerase enzymee. None of these answersThe dominant toxin from S. pneumoniae pneumolysinSelect one:O a. Is released from pneumococci only when they lyse and is not secretedO b. None of these answersO c. All of these answersO d. Is a potent membrane damaging toxinO e. Requires cholesterol to be toxic

Since you have asked multiple questions, we will solve the first question for you. If you want any…

Why is the temperature inside the thermal cycler lowered to 55°C during the PCR process? Select one: O a. To slow down the reaction halfway through O b. To speed up the reaction halfway through O c. To allow primers to bind to single-stranded DNA O d. To enhance the action of the Taq Polymerase enzyme O e. None of these answers

Why is the temperature inside the thermal cycler lowered to 55°C during the PCR process? Select one: O a. To slow down the reaction halfway through O b. To speed up the reaction halfway through O c. To allow primers to bind to single-stranded DNA O d. To enhance the action of the Taq Polymerase enzyme O e. None of these answers

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

See similar textbooks

Related questions

Q: I don't know what is that picture. I need help with the nameof label

A: The musculoskeletal or locomotor system is made by the collective contribution of bones, cartilage,…

Q: please help

A: Detailed explanation:2. What conclusions can you draw from lanes 2-5 (the PCR products)?Lanes 2-5…

Q: an you help me with this surgical instruments and equipment identification? I'm little confused to…

A: Surgery is the process of operating the patient. It involves cutting, suturing, and abrading.…

Q: Which is the BEST example of a community responding to an external factor? O A. Plants and animals…

A: An ecosystem is a geographic area where plants, animals, and other organisms, as well as weather and…

Q: what might be the possible cause of the mobile bay jubilee ? Can you write the answer in 7th grade…

A: Jubilee is the name given to a natural occurrence that happens on the shores of Mobile Bay on…

Q: Hello good day, I hope today has been kind to you. So I am having a problem answering this question…

A: According to our guideline we can't provide you the sources of the answer. Histology is the study of…

Q: What is the function of a cell wall? A to protect and support the cell B to perform different…

A: Cell wall is nonliving thing and it cover outermost layer of cell. It is permeable in nature…

Q: Z1 PS K CH 22

A: Cell cycle is of sequential events which is responsible for dividing the parent cell into two…

Q: z1 PS z2

A: Centrioles play a significant role in cell division. They help in formation of spindle fibers and…

Q: Can you help me see if I'm doing it correctly or incorrectly

A: Step 1: Step 2: Step 3: Step 4:

Q: Which is the site of filtration of blood and production of urine? -D AB C

A: The urinary system is comprised of specialized organs that play a crucial role in the production of…

Q: Help me to answer those questions please

A: Tonicity refers to osmotic pressure gradient. It is defined as relative concentration of solutes…

Q: is

A: The body part shown in the figure is the lower limb. Lower limbs is the anatomical term given to the…

Q: I don't know what is the picture please help

A: The human skeleton is the interior system of the human body. It is made out of around 270 bones upon…

Q: Question #8 Shown below are the chemical structures of several cofactors that are used in enzyme…

A: The names and properties of the coenzymes are as follows:

Q: Hi, can you please help me label this? Thank you

A: Skeletal muscles are muscle tissue attached to the body's bones. The skeletal muscle is made up of…

Q: Please help me and explain! Thanks

A: Explain what???

Q: composedof one pinktwo whte.and three ted fowered panales showaf yeurwork

A: Incomplete dominance occurs when a dominant allele, does not completely mask the effects of a…

Q: 1) Cephalic Phase the mouth The purpose of cephalic phase of digestion is to ea ten - prepare dnd…

A: Digestion is the process by which food is digested in the body with the help of various enzymes and…

Q: 14 13 of

A: It is a cochlear region. Parts are colored differently.

Concept explainers

Immune Disorders

The collaboration between the cells and proteins that work together as a defense system against infection comprises the immune system.Each of them performs a variety of tasks to recognize and/or react against a foreign body.

Question

thank you!!!

Why is the temperature inside the thermal cycler lowered to 55°C during the PCR process?
Select one:
O a. To slow down the reaction halfway through
O b. To speed up the reaction halfway through
O c. To allow primers to bind to single-stranded DNA
O d. To enhance the action of the Taq Polymerase enzyme
e. None of these answers
The dominant toxin from S. pneumoniae pneumolysin
Select one:
O a. Is released from pneumococci only when they lyse and is not secreted
O b. None of these answers
O c. All of these answers
O d. Is a potent membrane damaging toxin
O e. Requires cholesterol to be toxic

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1 Introduction: -

VIEW

Step 2 Explanation for the correct option: -

VIEW

Step 3 Explanation for incorrect options: -

VIEW

Step by step

Solved in 3 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

Competent E. coli cells were transformed with the pGLO plasmid. These transformed cells were then allowed to grow on two different plates: 1) a plate containing LB/AMP and 2) another plate containing LB/AMP/ARA. In which plate would you observe both phenotypic and genotypic changes? Briefly justify your answer. Edit View Insert Format Tools Table 12pt v Paragraph v BIUA e T?v 田 D2
Explain why a positive control and negative control are included in PCR experiments. Explain the three steps involved in each cycle of polymerase chain reaction.Why is loading dye added to the DNA sample for gel electrophoresis? Explain the function of the following components in a PCR reaction:− Primer, dNTP, MgCl, Taq polymerase, buffer.
Single stranded DNA fragment of M13 phage DNA shown was mixed with the following primers P1: AGTAG AATTGATGCC ACCTT; P2: TGT CTG GAA GTT TCA TTC CA and incubated in a thermal cycler in the presence of the four dNTPs and Taq DNA polymerase under the PCR conditions.AATGCTACTA CTATTAGTAG AATTGATGCC ACCTTTTCAG CTCGCGCCCC ATAGCTAAAC AGGTTATTGA CCATTTGCGA AATGTATCTA ATGGTCAAAC CGTTCGCAGA ATTGGGAATC AACTGTTACA TGGAATGAAA CTTCCAGACA What is the length of the PCR product? What is the final concentration of PCR product after 10 cycles if the starting template concentration was 1 nM?
Match the PCR sample with the predicted result on the agarose gel. 1) Sample from individual homozygous for their PV92 locus with the Alu insert. Five bands of 100, 200 500, 700 and 1000bp Single band of 300 bp Two bands one of 300 bp and one of 641bp Tow bands one of 641 bp and one of 941 bp No Bends Single band of 941 bp Single band of 641bp 2) Sample from individual homozygous for their PV92 locus without the Alu insert. Five bands of 100, 200 500, 700 and 1000bp Single band of 300 bp Two bands one of 300 bp and one of 641bp Tow bands one of 641 bp and one of 941 bp No Bends Single band of 941 bp Single band of 641bp 3) Sample from individual heterozygous for their PV92 locus having the Alu insert. Five bands of 100, 200 500, 700 and 1000bp Single band of 300 bp Two bands one of 300 bp and one of 641bp Tow bands one of 641 bp and one of 941 bp No Bends Single band of 941 bp Single band of 641bp 4) Negative control sample. Five bands of 100, 200 500, 700 and 1000bp…
FIGURE 2 shows the only suitable DNA restriction site in a plasmid DNA vector that can be cleaved. 5'.GTAATCCGGATCCGAAATCCCCCGG... 3' 3.CATTAGGCCTAGGCTTTAGGGGGCCC... 5 FIGURE 2 Identify the most suitable enzymeto be used as a restriction enzyme forthis purpose. O Ligase O Smal O EcoRI О ЕсoRV
Before Taq polymerase or Pfu polymerase were available, scientists used regular E. coli polymerase. Explain what was the hassle involved in using this polymerase for PCR, and then why the Taq or Pfu polymerase offered a solution to this problem. (Clearly address both parts of this question.)
In PCR amplification reaction, 5 mM EDTA was erroneously added to your final reaction, and you did not get any products. What is a possible explanation for this (based on the properties of EDTA)?
In PCr amplification If your negative control would have shown a different bp band that what was expected, what can be a possible explanation?
Perform the Megaprimer PCR procedure (in scheme A). Indicates the number of Primers required in the protocol and marks their location according to the location of the nucleotide to be mutated (the X represents the nucleotide to be mutated). A)
Pictured below is a map of the pBR322 plasmid vector. If a DNA fragment is inserted into the Sal I site, what can be expected about the growth coli transformants containing the recombinant plasmid? EcoRI Pstl Ampicillin resistance (AmpⓇ) Origin of replication (ori) d. All of the above are true pBR322 (4,361 bp) Pvull BamHI Tetracycline resistance (TetⓇ) Sall a. The transformants should grow on nutrient agar containing ampicillin and tetracycline. b. The transformants should be able to grow on nutrient agar without antibiotics. c. The transformants should be able to grown on nutrient agar containing ampicillin
Examine the pGLO plasmid DNA solution with the UV lamp. Note your observations. Using a micropipettor, withdraw 10 ul of plasmid and mix it into the cell suspension of the +PGLO tube by pipetting up and down three times. Close the tube and return it to the rack on ice. Also close the -PGLO tube. Do not add plasmid DNA to the -pGLO tube. Why not?
Evaluation of PCR product electrophoresed on 0.8% agarose gel shows non-specific bands. The appropriate modification for the next PCR reaction is to: a) increase the concentration of Taq polymerase b) increase the number of PCR cycles c) decrease the template denaturation temperature d) reduce the concentration of primers