Which would be more likely to cause illness and why—a water sample that tested positive for fecal coliforms or one that tested positive for E. coli O157:H7?
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Which would be more likely to cause illness and why—a water sample that tested positive for fecal coliforms or one that tested positive for E. coli O157:H7?
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- Savlon Extra” disinfectant is known to exhibit good antimicrobial activity against Shigella dysenteriae. At 20oC a concentration of 5% v/v kills 2.0 x106 Sh. dysenteriae cells in 15 minutes. At 25oC the organisms were killed in 5 minutes. a) Calculate the temperature coefficient of Savlon Extra b). How long will it take the same concentration to kill the same number of Sh. dysenteriae cells at 30oC?t /g = (Log Nt – Log N0) /0.301 10 mL of a culture of Enterobacter at 104 cells/mL is introduced into 990 mL of the same broth at the same temperature (37° C) with adequate mixing. It is 8 p.m. the night before class on Tuesday at 11 a.m. Doubling time for this species is 40 minutes. Will the culture reach Stationary Phase before class?What will happen to the color of the Coomassie reagent as more protein is added? Group of answer choices A) It will turn more Blue B) It will turn more Green C) It will turn more Red
- Flasks A and B contain broth, and are inoculated with an identical number of bacteria that are facultative anaerobes. Flask A contains a triglyceride lipid as the sole nutrient source, and Flask B contains a monosaccharide called fructose as the sole nutrient source. Both flasks are incubated at 30°C with aeration (oxygen is added to the broth). Assume the bacteria are capable of catabolism of both fructose and triglycerides. Mark all the correct statements pertaining to these cultures. A. Under anaerobic conditions, cells in flask A will produce more ATP B. Cells from flask B will produce more carbon dioxide C. Cells from flask B will produce more ATP D. Flask A will have the greater cell mass E. Cells from flask A will produce more ATP F. Cells from flask A will produce more carbon dioxide G. Flask B will have the greater cell mass H. Under anaerobic conditions, cells in flask B will produce more ATPIf coliforms were present on a plated sample, would you know with certainty that the coliforms are disease-causing? Why or why not?In the multiple fermentation tube technique does the absence of gas formation after 24 hours constitute a negative presumptive test for coliform bacteria? Explain.
- An otic product was subjected to AET and tested with the challenge microorganisms. An initial concentration of 2 x 10^5 cfu/mL of Escherichia coli was computed. Compute for the log reduction from the initial calculated count if the cfu/mL obtained is 1.2 x 10^4 at 7 days.if the average weight of content is 275 mg, Calculate the permissible limit (first) and the rejection limit (second) for a batch of capsulesA stool specimen of a patient with severe diarrhea was cultured in a series of specialized media for isolation of enteric organisms. The cultures yielded three isolates that were species of Salmonella, Shigella, and Escherichia. Explain why the Hydrogen sulfide production test would be diagnostically significant.
- Do the results from a confirmed coliform test represent total or faecal coliform and why?If you unfortunately ingested five (5) E. coli cells O157:H7 at 9:00 AM, May 27, how many cells would be present by 11:00 PM, May 28. E. coli generation time is 20 minutes. (Disregard stomach acid effect)In your own words, explain how you determine the susceptibility for tooth decay for individuals using the Snyder test? ( your answer should refer to lactic acid production, pH indicator or color change and time component)