Which type of organism (obligate anaerobe, microaerophile, facultative anaerobe, aerotolerant anaerobe) would most likely be affected negatively by the use of old media? Which type of organism (obligate anaerobe, microaerophile, facultative anaerobe, aerotolerant anaerobe) would most likely be affected positively by the use of old media? Which type of organism (obligate anaerobe, microaerophile, facultative anaerobe, aerotolerant anaerobe) would be least affected by old media?

Give a microbial species or microbial group that could be involved in the following: Alcohol production in grape juice? Spoilage of vacuum packaged chicken sausage stored at 2c? Spoilage of strawberries? Scombroid fish poisoning? Spoilage of honey?

How do microbes respond to high pressure? To ionizing radiation? To UV light? What populations are resistant to these conditions?

As a Microbiologist working on a bioremediation project, you are torn between 2 choices for remediation of soil contaminated with jet fuel. Your first option is to fertilize the soil with nitrogen and phosphorus for possible promotion of growth hydrocarbon-degrading microorganisms. For your second option, there is a possibility of using a new commercial microbial inoculum containing hydrocarbon-degrading microorganisms, nutrients and surfactants. What information will you need to decide on the best approach?