Which question MOST likely may have led to the development of recombinant DNAtechnology?Can human genes beintegrated intobacterial DNA so12CanCan DNA be cut intofragments byrestriction enzymesCan human genes beintroduced into theundifferentiatedcells be used tocells of people withgenetic disorders using repair parts of thebacteria can copy thegenes and producetheir proteins?and then separate intounique patterns?a virus?body?Sign outV O 9:20Music offZoom In100eLenovobacks&248.56.e

Recombinant DNA technology DNA molecules that are extracted from different sources and chemically…

Which question MOST likely may have led to the development of recombinant DNA technology? Can human genes be integrated into bacterial DNA so bacteria can copy the genes and produce their proteins? 2 Can Can DNA be cut into Can human genes be fragments by restriction enzymes introduced into the undifferentiated cells of people with genetic disorders using repair parts of the cells be used to and then separate into unique patterns? a virus? body?

Which question MOST likely may have led to the development of recombinant DNA technology? Can human genes be integrated into bacterial DNA so bacteria can copy the genes and produce their proteins? 2 Can Can DNA be cut into Can human genes be fragments by restriction enzymes introduced into the undifferentiated cells of people with genetic disorders using repair parts of the cells be used to and then separate into unique patterns? a virus? body?

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter17: Metabolism: An Overview

Section: Chapter Questions

Problem 13P: What Art the Features of the Series of -omes? Define the following terms: a. Genome b. Transcriptome...

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The way that PCR amplifi es DNA is similar to the doubling in a population of growing bacteria; a single DNA strand is used to synthesize 2 DNA strands, which become 4, then 8, then 16, etc. If a complete cycle takes 3 minutes, how many strands of DNA would theoretically be present after 10 minutes? After 1 hour?
Griffith, in his 1928 experiments, demonstrated that bacterial strains could be genetically transformed. The evidence that DNA was the transforming principle responsible for this phenomenon came later. What was the key experiment that Avery, MacCleod, and McCarty performed to prove that DNA was responsible for the genetic change from rough cells into smooth cells? Pls help explain this question asap!!
To amplify a section of DNA using the polymerase chain reaction (PCR), all you need to load into the tube is 1) a buffer solution, 2) the DNA you want to amplify, 3) some DNA nucleotides, 4) a polymerase (like Taq polymerase), and O an RNA polymerase a set of forward and reverse primers some phospholipids for a cell membrane some ribosomes
What is complementary base pairing? Be able to figure out the nucleotide sequence of the 2nd strand of DNA if you’re given the first (as happens when DNA replicates); or the RNA sequence that would pair with a DNA sequence (as happens during transcription). What is Biotechnology? Recombinant DNA? What is a restriction enzyme and how is it used to make recombinant DNA? What is a transgenic animal? Transgenic crops? What is Forensics? Why are the following things important in forensic (or medical genetic) testing: PCR? STRs? What is CODIS? What is a multifactorial trait? A polygenic trait? Some examples of each? How do they differ from Mendelian traits? What is cancer? How do cancer cells differ from normal cells? Why is control of the cell cycle crucial? Genetic influences in developing cancer: what is an oncogene? a tumor suppressor? Difference between a benign and a malignant tumore? Metastasis?
On further analysis of the DNA described in conceptual questionC21, you discover that the triplex DNA in this alien organism iscomposed of a double helix with a third strand wound within themajor groove (just like the DNA in Figure shown). How would youpropose that this DNA is able to replicate itself? In your answer,be specific about the base-pairing rules within the double helixand which part of the triplex DNA would be replicated first.
Part 3. Restriction Enzymes 1) Consider the sequence of DNA given below and answer the following questions 5’ ATTGAGGATCCGTAATGTGTCCTGATCACGCTCCACG 3’ 3’ TAACTCCTAGGCATTACACAGGACTAGTGCGAGGTGC 5’ a) You cut the sequence of DNA shown above using BamHI (see table 19.1 from the text). How many fragments of DNA would you expect to result from this restriction digest? b) If you cut the sequence of DNA shown above using BclI (recognition sequence = 5’ TGATCA 3’, enzyme cuts after the first T) instead of BamHI how many fragments do you expect? 2) For each given sequence/restriction enzyme pair, determine how many pieces of DNA would result form the digest and indicate whether those pieces would have blunt or sticky ends. NOTE: in the given recognition sites, the dash represents where the cut is made. a) HpaI, recognizes 5’ GTT – AAC 3’ 5’ GGATGTTAACAATCTCTACGGGTTAACACCCTTGGGTTAACATCCGCGG 3’ 3’ CCTACAATTGTTAGAGATGCCCAATTGTGGGAACCCAATTGTAGGCGCC 5’ Number of fragments of DNA:…
Calculate the number of times the DNA of a modern E. coli cell has beencopied accurately since its earliest bacterial precursor cell arose about 3.5billion years ago. Assume for simplicity that over this time period, E. coli hasundergone, on average, one cell division every 12 hours (this is anoverestimate for modern bacteria, but probably an underestimate for ancientbacteria).
Which of the following statements regarding DNA Synthesis at the ends of linear chromosomes is true? Because DNA polymerase has proofreading capablities, replication at the ends of linear chromosomes is usually error-free, and telomerase, an enzyme composed of both RNA and protein, simply ensures that any incorporated errors are corrected. O DNA synthesis at the ends of linear chromosomes is a problem also observed in prokaryotic cells. Ov. Cancer in humans is associated with a reduction in the activity of telomerase. y Telomerase, an enzyme composed of both RNA and protein. fünctions by cleaving the 3 overhangs that are left by DNA polymerase.
Explain why errors in DNA Replication are rare events in cells? should be an original thought, not Google’s. dont use others answers few sentences
Why is E. coli not the best species to use in the lab for DNA cloning and manipulation experiments? (even though we have used this bacteria the most for this purpose!) It grows slowly It has too many chromosomes and plasmids It is not naturally transformable It grows at a very high temperature at which DNA is not stable How are the two strands of double stranded DNA held together Covalent bonds between phosphate groups Hydrogen bonding between bases van der Waal interactions Duct tape Which of the following is true about double stranded DNA One strand has two 5' ends and the other has two 3' ends The strands are identical and completely symmetric Each strand has a 5' end and a 3' end Double stranded DNA contains NO phosphate groups True or False. The PCR test for the coronavirus includes and extra step to convert RNA to DNA. True False
Cells have only one fundamental wayof replicating DNA but many differentways of repairing it. Are there stillother, undiscovered ways that cellshave for repairing DNA?
What will be the newly synthesized DNA from the template given? DNA Template 3 - CGGATGCCCGTATAC-5 O 3- GCCTACGGGCATATG -5 O 5-GCCTACGGGCATAAG -3 O 5- GCCTACGGGCATATG-3 O3-CGGATGCCCGTATAC -5