Which amino acids would be expected to produce a similar sickling effect if substituted for Val at position 6? O A. leucine B. lysine Oc. arginine ID. aspartic acid OE. isoleucine

a. Identify the type of mutation shown below. b. How many amino acids are affected? c. What type of impact will this mutation have on the functionality of the protein? Second Letter UUU UUC UUA JUG UCu uCC UCA UCG UAU UAC UGU UOC Phe Tyr Cys Ser UAA Stop UGA Stop UAG Stop UGG Trp Leu cu cc Pro CUU COU CAU His CAC CUC CUA Leu Arg CCA Cca CGA CUG CAA Cin CAG AGU AGC AGA Arg AAU AUU AUC le AUA AUG Met ACO ACU ACC ACA Asn Ser AAC The MA AAG Lys AGG CAL KAC GUU Aep GUC Val CUA OCC Ala OCA GA Clu GAG Oly GOA GUG GGG Original DNA strand G AC 1. G. G. Mutated DNA strand GA Third letter

complete the sentence In the Sanger method, the nitrogen from the tripeptide (give the name of the tri-peptide using the one-letter-codes of the 3 amino acids forming it, no spaces or punctuation between letters. eg. ABC) (.       ) shown above acts as a (           )

UCAGUCAGUOPODOTO E GFL A Alanine GU STOP Tyrosine A Stop C STOP V A Valine GU Cysteine G Stop G Tryplophan Arginine G A C A U Leucine Serine A UG Lysine Proline Asparagine GACUGACU H. MIR START Glycine Serine Leucine Glutamic Phenyl acid Aspartie acid Histidine RSK Glutamine Arginine Methionine Isoleucine Threonine