When subculturing some fibroblast primary cell culture; you encountered a problem that the cells were not coming out of the flask bottom and stuck there. Which of the following will you do to troubleshoot the issue? Make a fresh solution of EDTA and Antibiotics Make a fresh solution of Trypsin and Calcium Chloride O Make a fresh solution of Trypsin and EDTA O Make a fresh solution of Collagenase and Calcium Chloride

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A:

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.