When subculturing some fibroblastprimary cell culture; you encountered aproblem that the cells were not comingout of the flask bottom and stuck there.Which of the following will you do totroubleshoot the issue?Make a fresh solution of EDTA andAntibioticsO Make a fresh solution of Trypsin andCalcium ChlorideO Make a fresh solution of Trypsin and EDTAMake a fresh solution of Collagenase andCalcium Chloride The phenomenon in which a chemicalabsorbs light at one wavelength and emitsit at a specific and shorter wavelength iscalled:O Epi-fluorescenceO Phase contrastOFluorescenceO None of the answer choice is correctDifferential interference contrast

During the process of cell adhesion, cells interact and link with one another via specialized…

When subculturing some fibroblast primary cell culture; you encountered a problem that the cells were not coming out of the flask bottom and stuck there. Which of the following will you do to troubleshoot the issue? Make a fresh solution of EDTA and Antibiotics Make a fresh solution of Trypsin and Calcium Chloride O Make a fresh solution of Trypsin and EDTA O Make a fresh solution of Collagenase and Calcium Chloride

When subculturing some fibroblast primary cell culture; you encountered a problem that the cells were not coming out of the flask bottom and stuck there. Which of the following will you do to troubleshoot the issue? Make a fresh solution of EDTA and Antibiotics Make a fresh solution of Trypsin and Calcium Chloride O Make a fresh solution of Trypsin and EDTA O Make a fresh solution of Collagenase and Calcium Chloride

Aquaculture Science

3rd Edition

ISBN:9781133558347

Author:Parker

Publisher:Parker

Chapter2: Aquatic Plants And Animals

Section: Chapter Questions

Problem 2KA

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Detergents that are good micelle formers often have strongantibacterial properties. Considering the structure of thecell membrane, suggest a reason for this antibacterialaction.
A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…
A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…
A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…
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