When subculturing some fibroblast primary cell culture; you encountered a problem that the cells were not coming out of the flask bottom and stuck there. Which of the following will you do to troubleshoot the issue? Make a fresh solution of EDTA and Antibiotics Make a fresh solution of Trypsin and Calcium Chloride O Make a fresh solution of Trypsin and EDTA O Make a fresh solution of Collagenase and Calcium Chloride

D. OSMOSIS WITH LIVING PLANT CELLS C. a. Procedure This experiment is designed to demonstrate the principles of osmosis with living plant tissues. Using a corkborer, bore two equal-sized cylinders of freshly cut raw potato. Feel them and note the degree of firmness. Place one potato piece in a culture dish containing enough five percent salt solution to cover the potato completely. Place the second potato piece in a culture dish containing the same amount of distilled water. After about 20 minutes, examine both pieces of potato and note any changes in their degree of firmness. b. Results SOLUTION AROUND POTATO 5% Salt Distilled water doesn't Molle 78 FIRMNESS OF POTATO AFTER 20 MINUTES soggy Firm Questions 1. Give an explanation for your results with the 5% salt solution.

A dialysis bag that contains 20% sucrose solution is placed in a beaker with a 5% sucrose solution. Which solution is hypotonic? cannot be determined    both solutions     the 20% sucrose solution    the 5% sucrose solution

What cell type is this? Explain why Fibroblasts Epithelial cells Lymphocytes Macrophages the photo is of stained mammalian cellsthat are under under a compound light microscope at 400x total magnification. the cells were treated with cell fixing agent, methylene blue stain, and eosin stain.

Cell Transport Method Passive or Active Process? Is process used to molecules move DOWN their concentration gradient, AGAINST their concentration gradient, or to move LOTS of a molecule at once? Is Definition

An image was captured using a microscope using a laser to excite fluorochromes that were bound to the cell's cytoskeleton. What type of microscope was utilized to capture this image? O Fluorsecent O scanning electron O darkfield O confocal

The motor proteit v transports cargo in the direction, carrying it from the soma to the terminals. microtubule GABA neurofilament sodium myelin kinesin connexin dynein

In your lab you are trying to culture skin tissue for use in transplant experiments. You’ve encountered a problem because the cells seem to dissociate after only one day in culture. What might be one solution to your problem (i.e., what might you add to your medium to get your cells to stick together)? Why?

Hemidesmosomes and focal adhesions link the inside of a cell to a substrate. True False