When separating your organic and aqueous layer and collecting organic for the final time, is it better to take some of the aqueous layer than the organic layer to ensure collection of all of the organic layer, or to leave some of the organic layers with the aqueous layer to ensure collection of all of the aqueous layer? Explain why.
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When separating your organic and aqueous layer and collecting organic for the final time, is it better to take some of the aqueous layer than the organic layer to ensure collection of all of the organic layer, or to leave some of the organic layers with the aqueous layer to ensure collection of all of the aqueous layer? Explain why.
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- What characteristic of agarose makes it useful for electrophoresis? It is more dense than water and an electrical current can pass through it. The agarose gel contains microscopic pores which act as molecular sieves. The sieving properties of the gel influences the rate at which molecules migrate. It is jelly like and clear so that you can see your molecules move through it.You fill a bag made of semipermeable membrane with a .5 mol/litre solution of sugar, seal it and immerse it into a .3mol/litre solution of salt and water. Which description best fits what will happen. 1) The environment is hypertonic to the contents, so the bag will shrink as water leaves the bag. 2)The environment is hypotonic to the contents, so the bag will expand as water enters the bag. 3) The environment is hypertonic to the contents, so the bag will expand as water enters the bag. 4) The environment is hypotonic to the contents, so the bag will shrink as water leaves the bag.Which of the following techniques can best separate a protein from other proteins on the basis of size? Electrophoresis in polyacrylamide gels containing SDS lon-exchange chromatography Dialysis using a membrane . Isoelectric focusing Differential centrifugation
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- If the agar instructions say to mix 50 grams of agar in 1 liter of water, how many grams of agar would you mix into 100 milliliters of water to get the proper concentration of agar?Scenario You have a polypeptide chain that is linked by disulfide bond. If beta-mercaptoethanol is applied, there is no disulfide bond anymore. What has happened to the polypeptide chain after the detergent abovehas been applied. Does it increase, decrease or remain the same?What is the advantage of the doing cell wall isolation, determining composition by the use of Matrix Polysaccharide composition and Crystalline Cellulose content?
- Why do we use sucrose solution when we observe plant cells? What explanation please..The term "turbidity" in microbiology refers to: a cloudy appearance in broth, meaning microorganisms did grow a cloudy appearance in broth, meaning microorganisms did not grow a clear appearance in broth, meaning microorganisms did not grow the clump of cells at the top of a broth a clear appearance in broth, meaning microroganisms did growKey tips for extraction different type of molecules; lipids, proteins, sugar, nucleus acid