What sizes of pieces should you get if you cut with: Pst1 Xba1 Sac1

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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What sizes of pieces should you get if you cut with:

  1. Pst1
  2. Xba1
  3. Sac1
Clal (2)
AfllI (301)
EcoRV (386)
AraC
ORF frame 3
pBRrevBam_primer
PGEX_3_primer
Nrul (708)
ara02
Agel (1074)
ara01
CAP BS
PBAD_fwd_primer
5371
4924
449
Aral112
PBR322_origin
ARA_promoter
T7 transl_en_RBS
4476
896
Sequence
5371 bp
4029
1344
3581
1791
f1_origin
Nhel (1345)
GFP_R_primer
GFPfusionREV_primer
Ncol (1510)
Mscl (1515)
GFP (0 - 695)
3134
2239
2686
ORF frame 1
Asel (3248)
Fspl (3200)
ORF frame 2
Ampicillin
Xhol (1765)
BstBI (1966)
GFP_F_primer
EcoRI (2063)
Kpnl (2079)
Xmal (2079)
Smal (2081)
Xbal (2090)
PBAD_rev_primer
pTrcHis_rev_primer
rrnB_terminator
rrnB_T1 terminator
rrnB T2 terminator
AmpR_promoter
Feature Options-
Restriction Enzymes
Unique
2-cutters Ohide all
Transcribed Image Text:Clal (2) AfllI (301) EcoRV (386) AraC ORF frame 3 pBRrevBam_primer PGEX_3_primer Nrul (708) ara02 Agel (1074) ara01 CAP BS PBAD_fwd_primer 5371 4924 449 Aral112 PBR322_origin ARA_promoter T7 transl_en_RBS 4476 896 Sequence 5371 bp 4029 1344 3581 1791 f1_origin Nhel (1345) GFP_R_primer GFPfusionREV_primer Ncol (1510) Mscl (1515) GFP (0 - 695) 3134 2239 2686 ORF frame 1 Asel (3248) Fspl (3200) ORF frame 2 Ampicillin Xhol (1765) BstBI (1966) GFP_F_primer EcoRI (2063) Kpnl (2079) Xmal (2079) Smal (2081) Xbal (2090) PBAD_rev_primer pTrcHis_rev_primer rrnB_terminator rrnB_T1 terminator rrnB T2 terminator AmpR_promoter Feature Options- Restriction Enzymes Unique 2-cutters Ohide all
Cutters | Linear Digest | Circular Digest
SgrAl (5307)
Afel (5225)
Pcil (4748)
Clal (2)
Nsil (9)
Afll (301)
EcoRV (386)
BssHII (670)
4924
449
Nrul (708)
Agel (1074)
4476
896
Sequence
5371 bp
4029
1344
3581
1791
Psil (3896)
Nhel (1345)
Bmtl (1349)
Ncol (1510)
Mscl (1515)
BsrGI (1620)
Xhol (1765)
BstBI (1966)
3134
2239
2686
Asel (3248)
Fspl (3200)
Pvul (3054)
Scal (2942)
EcoRI (2063)
Acc651 (2075)
Kpnl (2079)
Xmal (2079)
Smal (2081)
Xbal (2090)
Sbfl (2106)
Sphl (2112)
Feature Options-
Restriction Enzymes VUnique V2-cutters OAII ONon-cutters
Transcribed Image Text:Cutters | Linear Digest | Circular Digest SgrAl (5307) Afel (5225) Pcil (4748) Clal (2) Nsil (9) Afll (301) EcoRV (386) BssHII (670) 4924 449 Nrul (708) Agel (1074) 4476 896 Sequence 5371 bp 4029 1344 3581 1791 Psil (3896) Nhel (1345) Bmtl (1349) Ncol (1510) Mscl (1515) BsrGI (1620) Xhol (1765) BstBI (1966) 3134 2239 2686 Asel (3248) Fspl (3200) Pvul (3054) Scal (2942) EcoRI (2063) Acc651 (2075) Kpnl (2079) Xmal (2079) Smal (2081) Xbal (2090) Sbfl (2106) Sphl (2112) Feature Options- Restriction Enzymes VUnique V2-cutters OAII ONon-cutters
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